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Production of poly(adenosine diphosphate-ribose) polymerase-1 : development of an efficient production and purification protocol

Knight, Matthew (2003) Production of poly(adenosine diphosphate-ribose) polymerase-1 : development of an efficient production and purification protocol. PhD thesis, Victoria University of Technology.

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Abstract

PARP-1 is a nuclear enzyme involved in a range of activities associated with DNA metabolism, playing a key role in maintaining the integrity of DNA and chromatin structure. As such, this enzyme is likely to provide a useful target when using a rationale drug design approach to develop pharmaceutical reagents including cancer therapeutics. A major obstacle to this work however is that our knowledge of the relationship between structure and function of PARP-1 is rather limited. Structure - function studies of enzymes typically require the application of techniques for producing reasonable quantities of high quality protein. To this end, this thesis describes the development of a rapid and relatively simple approach to producing and purifying PARP-1.

Item Type: Thesis (PhD thesis)
Uncontrolled Keywords: NAD-ADP-ribosyltransferase, Separation, Purification, enzymes, DNA, PARP-1
Subjects: FOR Classification > 0601 Biochemistry and Cell Biology
Faculty/School/Research Centre/Department > School of Engineering and Science
FOR Classification > 0304 Medicinal and Bimolecular Chemistry
Depositing User: VU Library
Date Deposited: 22 Aug 2010 23:51
Last Modified: 23 May 2013 16:45
URI: http://vuir.vu.edu.au/id/eprint/15621
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