Yeast-mediated preparation of I-PAC in an organic solvent
Kostraby, Margaret M and Smallridge, Andrew John and Trewhella, Maurice Arthur (2002) Yeast-mediated preparation of I-PAC in an organic solvent. Biotechnology and Bioengineering, 77 (7). pp. 827-831. ISSN 0006-3592Full text for this resource is not available from the Research Repository.
The yeast-mediated acyloin condensation of benzaldehyde and pyruvic acid to form l-PAC occurs in a petroleum spirit solvent system at room temperature with moderate conversion (30%) and high enantioselectivity (86%ee) after 24 h. The addition of a small amount of ethanol (0.5% mL) to the reaction mixture inhibits the formation of the side product benzyl alcohol and increases the conversion to l-PAC. Conducting the reaction using 13C-labeled pyruvate indicated that the pyruvate was incorporated into the l-PAC and that the excess pyruvate was converted into ethanol. Conducting the reaction at 5°C results in similar conversion but higher enantioselectivity.
|Uncontrolled Keywords:||I-PAC, yeast-mediated acyloin condensation, pyruvate|
|Subjects:||RFCD Classification > 290000 Engineering and Technology
Faculty/School/Research Centre/Department > School of Engineering and Science
|Depositing User:||Ms Phung T Tran|
|Date Deposited:||25 Feb 2009 11:16|
|Last Modified:||04 Jul 2011 05:16|
|ePrint Statistics:||View download statistics for this item|
|Citations in Scopus:||14 - View on Scopus|
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