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Short-term docosapentaenoic acid (22:5n-3) supplementation increases tissue docosapentaenoic acid, DHA and EPA concentrations in rats

Kaur, Gunveen, Begg, Denovan P, Barr, Daniel P, Garg, Manohar, Cameron-Smith, David and Sinclair, Andrew J (2010) Short-term docosapentaenoic acid (22:5n-3) supplementation increases tissue docosapentaenoic acid, DHA and EPA concentrations in rats. British Journal of Nutrition, 103 (1). pp. 32-37. ISSN 0007-1145 (print), 1475-2662 (online)

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Abstract

The metabolic fate of dietary n-3 docosapentaenoic acid (DPA) in mammals is currently unknown. The aim of the present study was to determine the extent of conversion of dietary DPA to DHA and EPA in rats. Four groups of male weanling Sprague–Dawley rats (aged 5 weeks) were given 50 mg of DPA, EPA, DHA or oleic acid, daily for 7 d by gavage. At the end of the treatment period, the tissues were analysed for concentrations of long-chain PUFA. DPA supplementation led to significant increases in DPA concentration in all tissues, with largest increase being in adipose (5-fold) and smallest increase being in brain (1·1-fold). DPA supplementation significantly increased the concentration of DHA in liver and the concentration of EPA in liver, heart and skeletal muscle, presumably by the process of retroconversion. EPA supplementation significantly increased the concentration of EPA and DPA in liver, heart and skeletal muscle and the DHA concentration in liver. DHA supplementation elevated the DHA levels in all tissues and EPA levels in the liver. Adipose was the main tissue site for accumulation of DPA, EPA and DHA. These data suggest that dietary DPA can be converted to DHA in the liver, in a short-term study, and that in addition it is partly retroconverted to EPA in liver, adipose, heart and skeletal muscle. Future studies should examine the physiological effect of DPA in tissues such as liver and heart.

Item Type: Article
Additional Information:

Accepted version has title: Short term DPA (22-5n-3) supplementation increases tissue DPA, DHA and EPA concentration in rats

Uncontrolled Keywords: metabolism, lipids, adipose, diet
Subjects: FOR Classification > 1101 Medical Biochemistry and Metabolomics
FOR Classification > 1111 Nutrition and Dietetics
Faculty/School/Research Centre/Department > Institute of Sport, Exercise and Active Living (ISEAL)
Depositing User: VUIR
Date Deposited: 21 Jan 2013 22:40
Last Modified: 14 Nov 2017 06:16
URI: http://vuir.vu.edu.au/id/eprint/21395
DOI: https://doi.org/10.1017/S0007114509991334
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Citations in Scopus: 57 - View on Scopus

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