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Effects of endurance training status and sex differences on Na+, K+-pump mRNA expression, content and maximal activity in human skeletal muscle

Murphy, Kate Tamarind and Aughey, Robert J and Petersen, Aaron C and Clark, Sally A and Goodman, Craig and Hawley, J. A and Cameron-Smith, David and Snow, R. J and McKenna, Michael J (2007) Effects of endurance training status and sex differences on Na+, K+-pump mRNA expression, content and maximal activity in human skeletal muscle. Acta Physiologica, 189 (3). pp. 259-269. ISSN 1748-1708

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Abstract

This study investigated the effects of endurance training status and sex differences on skeletal muscle Na+,K+-pump mRNA expression, content and activity. Methods: Forty-five endurance-trained males (ETM), 11 recreationally active males (RAM), and nine recreationally active females (RAF) underwent a vastus lateralis muscle biopsy. Muscle was analysed for Na+,K+-pump α1, α2, α3, β1, β2 and β3 isoform mRNA expression (real-time reverse transcription-polymerase chain reaction), content ([3H]-ouabain-binding site) and maximal activity (3- O-methylfluorescein phosphatase, 3- O-MFPase). Results: ETM demonstrated lower α1, α3, β2 and β3 mRNA expression by 74%, 62%, 70% and 82%, respectively, than RAM ( P < 0.04). In contrast, [3H]-ouabain binding and 3- O-MFPase activity were each higher in ETM than in RAM, by 16% ( P < 0.03). RAM demonstrated a 230% and 364% higher α3 and β3 mRNA expression than RAF, respectively ( P < 0.05), but no significant sex differences were found for α1, α2, β1 or β2 mRNA, [3H]-ouabain binding or 3- O-MFPase activity. No significant correlation was found between years of endurance training and either [3H]-ouabain binding or 3- O-MFPase activity. Significant but weak correlations were found between the number of training hours per week and 3- O-MFPase activity ( r = 0.31, P < 0.02) and between incremental exercise O2(peak) and both [3H]-ouabain binding ( r = 0.33, P < 0.01) and 3- O-MFPase activity ( r = 0.28, P < 0.03). Conclusions: Isoform-specific differences in Na+,K+-pump mRNA expression were found with both training status and sex differences, but only training status influenced Na+,K+-pump content and maximal activity in human skeletal muscle. Corrected by Corrigendum: Effects of endurance training status and sex differences on Na+, K+-pump mRNA expression, content and maximal activity in human skeletal muscle, Vol. 190, Issue 3, 261, 2007

Item Type: Article
Uncontrolled Keywords: exercise, endurance sports, gene, NA+, K+-pump, transcription, musculoskeletal system
Subjects: FOR Classification > 1106 Human Movement and Sports Science
Faculty/School/Research Centre/Department > School of Sport and Exercise Science
Depositing User: VUIR
Date Deposited: 27 Feb 2013 05:14
Last Modified: 29 Jan 2015 23:01
URI: http://vuir.vu.edu.au/id/eprint/21481
DOI: 10.1111/j.1748-1716.2006.01635.x
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Citations in Scopus: 10 - View on Scopus

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