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Transgenic mice expressing cre-recombinase specifically in retinal rod bipolar neurons

Zhang, Xinmei and Chen, Bai-Yu and Hoi-Lam, Alam Ng and Tanner, Julian A and Tay, David and So, Kwok-Fai and Rachel, Rivka A and Copeland, Neal G and Jenkins, Nancy A and Huang, Jian-Dong (2005) Transgenic mice expressing cre-recombinase specifically in retinal rod bipolar neurons. Investigative Ophthalmology & Visual Science, 46 (10). pp. 3515-3520. ISSN 1552-5783

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Abstract

PURPOSE. To establish a transgenic mouse line that expresses Cre-recombinase in retinal rod bipolar cells for the generation of rod bipolar cell-specific knockout mutants. METHODS. The IRES-Cre-cDNA fragment was inserted into a 173-kb bacterial artificial chromosome (BAC) carrying the intact Pcp2 gene, by using red-mediated recombineering. Transgenic mice were generated with the modified BAC and identified. The Cre-transgenic mice were crossed with ROSA26 and Z/EG reporter mice to detect Cre-recombinase activity. RESULTS. X-gal staining showed that strong Cre-recombinase activities were present in retinal inner nuclear layers and cerebellar Purkinje cells. Double staining with an anti-GFP antibody and an anti-PKCα antibody (specific for retinal rod bipolar cells) revealed that Cre-recombinase activity localized exclusively to the rod bipolar cells in the retina. CONCLUSIONS. A mouse BAC-Pcp2-IRES-Cre transgenic line that expresses Cre-recombinase in retinal rod bipolar neurons has been established. Because mutations in some ubiquitously expressed genes may result in retinal degenerative diseases, the mouse strain BAC-Pcp2-IRES-Cre will be a useful new tool for investigating the effects of retinal rod bipolar cell-specific gene inactivation.

Item Type: Article
Uncontrolled Keywords: night vision, scotopic conditions, outer plexiform layers, OPLs, rod photoreceptors, form synapses, rod bipolar cells, inner plexiform layers, IPLs, rod pathway, mammalian retina, rod photoreceptors, rod-connected neurons
Subjects: FOR Classification > 1109 Neurosciences
FOR Classification > 1113 Optometry and Ophthalmology
Faculty/School/Research Centre/Department > School of Sport and Exercise Science
Depositing User: VUIR
Date Deposited: 22 May 2013 04:05
Last Modified: 05 Mar 2015 05:50
URI: http://vuir.vu.edu.au/id/eprint/21758
DOI: 10.1167/iovs.04-1201
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Citations in Scopus: 9 - View on Scopus

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