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Role of calcium alginate and mannitol in protecting Bifidobacterium

Dianawati, Dianawati and Mishra, Vijay Kumar and Shah, Nagendra P (2012) Role of calcium alginate and mannitol in protecting Bifidobacterium. Applied and Environmental Microbiology, 78 (19). pp. 6914-6921. ISSN 0099-2240 (print) 1098-5336 (online)

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Abstract

Fourier transform infrared (FTIR) spectroscopy was carried out to ascertain the mechanism of Ca-alginate and mannitol protection of cell envelope components and secondary proteins of Bifidobacterium animalis subsp. lactis Bb12 after freeze-drying and after 10 weeks of storage at room temperature (25°C) at low water activities (aw) of 0.07, 0.1, and 0.2. Preparation of Ca-alginate and Ca-alginate-mannitol as microencapsulants was carried out by dropping an alginate or alginate-mannitol emulsion containing bacteria using a burette into CaCl2 solution to obtain Ca-alginate beads and Ca-alginate-mannitol beads, respectively. The wet beads were then freeze-dried. The aw of freeze-dried beads was then adjusted to 0.07, 0.1, and 0.2 using saturated salt solutions; controls were prepared by keeping Ca-alginate and Ca-alginate-mannitol in aluminum foil without aw adjustment. Mannitol in the Ca-alginate system interacted with cell envelopes during freeze-drying and during storage at low aws. In contrast, Ca-alginate protected cell envelopes after freeze-drying but not during 10-week storage. Unlike Ca-alginate, Ca-alginate-mannitol was effective in retarding the changes in secondary proteins during freeze-drying and during 10 weeks of storage at low aws. It appears that Ca-alginate-mannitol is more effective than Ca-alginate in preserving cell envelopes and proteins after freeze-drying and after 10 weeks of storage at room temperature (25°C).

Item Type: Article
Uncontrolled Keywords: ResPubID25551, bacteria, cells
Subjects: FOR Classification > 1101 Medical Biochemistry and Metabolomics
FOR Classification > 1108 Medical Microbiology
Faculty/School/Research Centre/Department > College of Health and Biomedicine
Depositing User: Yimin Zeng
Date Deposited: 21 Jan 2014 01:36
Last Modified: 13 Aug 2014 05:25
URI: http://vuir.vu.edu.au/id/eprint/23403
DOI: 10.1128/AEM.01724-12
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