Knockdown of Hop downregulates RhoC expression, and decreases pseudopodia formation and migration in cancer cell lines

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Willmer, Tarryn, Contu, Lara, Blatch, Gregory L and Edkins, Adrienne L (2013) Knockdown of Hop downregulates RhoC expression, and decreases pseudopodia formation and migration in cancer cell lines. Cancer Letters, 328 (2). pp. 252-260. ISSN 0304-3835

Abstract

The Hsp90/Hsp70 organising protein (Hop) is a co-chaperone that mediates the interaction of Hsp90 and Hsp70 molecular chaperones during assembly of Hsp90 complexes in cells. Formation of Hsp90 complexes is a key intermediate step in the maturation and homeostasis of oncoproteins and several hormone receptors. In this paper, we demonstrate that knockdown of Hop decreased migration of Hs578T and MDA-MB-231 breast cancer cells. Hop was identified in isolated pseudopodia fractions; it colocalised with actin in lamellipodia, and co-sedimented with purified actin in vitro. Knockdown of Hop caused a decrease in the level of RhoC GTPase, and significantly inhibited pseudopodia formation in Hs578T cells. Our data suggest that Hop regulates directional cell migration by multiple unknown mechanisms.

Item type Article
URI http://vuir.vu.edu.au/id/eprint/23417
Identification Number https://doi.org/10.1016/j.canlet.2012.09.021
Official URL http://www.sciencedirect.com/science/article/pii/S...
Subjects Current > FOR Classification > 1101 Medical Biochemistry and Metabolomics
Current > FOR Classification > 1108 Medical Microbiology
Current > Division/Research > College of Health and Biomedicine
Keywords ResPubID25532, ResPubID26659, Hop, cell migration, pseudopodia, RhoC, RNA interference
Citations in Scopus 21 - View on Scopus
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