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The simultaneous determination of vitamins A, E and β-carotene in bovine milk by high performance liquid chromatography–ion trap mass spectrometry (HPLC–MSn)

Plozza, Tim, Trenerry, V. Craige and Caridi, Domenico (2012) The simultaneous determination of vitamins A, E and β-carotene in bovine milk by high performance liquid chromatography–ion trap mass spectrometry (HPLC–MSn). Food Chemistry, 134 (1). pp. 559-563. ISSN 0308-8146

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Abstract

Bovine milk is considered to be a good dietary source of vitamins A, E and β-carotene. This paper describes a robust method for the simultaneous determination of these compounds using high performance liquid chromatography–ion trap mass spectrometry (HPLC–MSn) as the determinative step. The compounds were separated with a 5 μm Polaris 2.1 × 150 mm C18-A column and an aqueous-methanol mobile phase and the levels determined using the MS2 fragments (vitamin A, m/z 213 and m/z 199, vitamin E m/z 165 and β-carotene m/z 413). The HPLC–MSn was operated in the positive ion APCI mode. The method was validated using repeatability studies, duplicate analyses, recovery experiments, proficiency study data and comparison with previously validated high performance liquid chromatographic ultraviolet/visible (HPLC–UV/Vis) and fluorescence (HPLC–Fl) procedures used in our laboratory. Expanded measurement uncertainties were similar for HPLC–MSn, HPLC–UV/Vis and HPLC–Fl, e.g. vitamin A 45 μg/100 ml, HPLC–MSn ±8%, HPLC–UV ±9%; vitamin E 150 μg/100 ml, HPLC–MSn ±11%, HPLC–Fl ±9%; β-carotene 12 μg/100 ml, HPLC–MSn ±18%, HPLC–Vis ±21%.

Item Type: Article
Uncontrolled Keywords: ResPubID25500, quantification, vitamin A, vitamin E, β-carotene, bovine milk, liquid chromatography–ion trap mass spectrometry (HPLC–MSn)
Subjects: FOR Classification > 0908 Food Sciences
Faculty/School/Research Centre/Department > College of Science and Engineering
Depositing User: Yimin Zeng
Date Deposited: 10 Mar 2014 06:11
Last Modified: 25 Sep 2014 05:06
URI: http://vuir.vu.edu.au/id/eprint/23439
DOI: https://doi.org/10.1016/j.foodchem.2012.02.121
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Citations in Scopus: 34 - View on Scopus

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