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Adipophilin distribution and colocalisation with lipid droplets in skeletal muscle

Shaw, Christopher, Sherlock, Mark, Stewart, Paul M and Wagenmakers, Anton J. M (2009) Adipophilin distribution and colocalisation with lipid droplets in skeletal muscle. Histochemistry and Cell Biology, 131 (5). pp. 575-581. ISSN 0948-6143 (print) 1432-119X (online)

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Abstract

Intramyocellular lipids (IMCL) are stored as discrete lipid droplets which are associated with a number of proteins. The lipid droplet-associated protein adipophilin (the human orthologue of adipose differentiation-related protein) is ubiquitously expressed and is one of the predominant lipid droplet-proteins in skeletal muscle. The aim of this study was to investigate the subcellular distribution of adipophilin in human muscle fibres and to measure the colocalisation of adipophilin with IMCL. Muscle biopsies from six lean male cyclists (BMI 23.4 ± 0.4, aged 31 ± 2 years, W max 346 ± 8) were stained for myosin heavy chain type 1, IMCL, adipophilin and mitochondria using immunofluorescence and viewed with widefield and confocal fluorescence microscopy. The present study shows that like IMCL, the adipophilin content is ~twofold greater in type I skeletal muscle fibres and is situated in the areas between the mitochondrial network. Colocalisation analysis demonstrated that 61 ± 2% of IMCL contain adipophilin. Although the majority of adipophilin is contained within IMCL, 36 ± 4% of adipophilin is not associated with IMCL. In conclusion, this study indicates that the IMCL pool is heterogenous, as the majority but not all IMCL contain adipophilin.

Item Type: Article
Uncontrolled Keywords: ResPubID26399, adipose-differentiation related protein, ADRP, proteins, intramyocellular lipids, IMCL, PAT-proteins, fluorescence microscopy, skeletal muscle
Subjects: FOR Classification > 1101 Medical Biochemistry and Metabolomics
Faculty/School/Research Centre/Department > Institute of Sport, Exercise and Active Living (ISEAL)
Depositing User: Ms Phung.T Tran
Date Deposited: 22 Jul 2014 03:35
Last Modified: 16 Jan 2015 05:30
URI: http://vuir.vu.edu.au/id/eprint/23610
DOI: https://doi.org/10.1007/s00418-009-0558-4
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Citations in Scopus: 36 - View on Scopus

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