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No decline in skeletal muscle oxidative capacity with aging in long-term calorically restricted rats: effects are independent of mitochondrial DNA integrity

Baker, David J, Betik, Andrew C, Krause, Daniel J and Hepple, Russell T (2006) No decline in skeletal muscle oxidative capacity with aging in long-term calorically restricted rats: effects are independent of mitochondrial DNA integrity. Journals of Gerontology Series A: Biological Sciences and Medical , 61A (7). pp. 675-684. ISSN 1079-5006 (print) 1758-535X (online)

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Abstract

We investigated if calorie restriction (CR) preserved skeletal muscle oxidative capacity with aging after accounting for life span extension by CR, and determined if mitochondrial content, mitochondrial DNA integrity, and peroxisome proliferator-activated receptor gamma coactivator- 1 a (PGC-1 a ) were involved. Ad libitum-fed (AL) and CR animals representing young adult, late middle age, and senescence were studied. Whereas citrate synthase and complex IV activities were lower in plantaris and gastrocnemius muscle of young adult CR animals, in contrast to the 15%–40% decline in senescent AL animals, there was no decline with aging in CR animals. There was no decline in citrate synthase protein in gastrocnemius with aging in either group, suggesting that CR preserves oxidative capacity with aging by protecting mitochondrial function rather than content. This protection was independent of mitochondrial DNA damage between groups. However, there was a slower decline in PGC-1 a gene expression with aging in CR versus AL animals, suggesting a better maintenance of mitochondrial biogenesis with aging in CR animals.

Item Type: Article
Uncontrolled Keywords: aging, oxygen uptake, sarcopenia, mitochondria, endurance training, low calorie diet, muscles, peroxisomes, biochemistry
Subjects: FOR Classification > 1102 Cardiorespiratory Medicine and Haematology
Faculty/School/Research Centre/Department > College of Sports and Exercise Science
Depositing User: VUIR
Date Deposited: 08 Jan 2014 06:42
Last Modified: 13 Jan 2014 01:14
URI: http://vuir.vu.edu.au/id/eprint/24204
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Citations in Scopus: 69 - View on Scopus

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