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Cofilin Interacts with ClC-5 and Regulates Albumin Uptake in Proximal Tubule Cell Lines

Hryciw, Deanne and Wang, Yinghong and Devuys, Olivier and Pollock, Carol and Poronnik, Philip and Guggino, William (2003) Cofilin Interacts with ClC-5 and Regulates Albumin Uptake in Proximal Tubule Cell Lines. Journal of Biological Chemistry, 278 (41). pp. 40169-40176. ISSN 0021-9258

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Abstract

Receptor-mediated endocytosis is a constitutive high capacity pathway for the reabsorption of proteins from the glomerular filtrate by the renal proximal tubule. ClC-5 is a voltage-gated chloride channel found in the proximal tubule where it has been shown to be essential for protein uptake, based on evidence from patients with Dent’s disease and studies in ClC-5 knockout mice. To further delineate the role of ClC-5 in albumin uptake, we performed a yeast two-hybrid screen with the Cterminal tail of ClC-5 to identify any interactions of the channel with proteins involved in endocytosis. We found that the C-terminal tail of ClC-5 bound the actin depolymerizing protein, cofilin, a result that was confirmed by GST-fusion pulldown assays. In cultured proximal tubule cells, cofilin was distributed in nuclear, cytoplasmic, and microsomal fractions and co-localized with ClC-5. Phosphorylation of cofilin by overexpressing LIM kinase 1 resulted in a stabilization of the actin cytoskeleton. Phosphorylation of cofilin in two proximal tubule cell models (porcine renal proximal tubule and opossum kidney) was also accompanied by a pronounced inhibition of albumin uptake. This study identifies a novel interaction between the C-terminal tail of ClC-5 and cofilin, an actin-associated protein that is crucial in the regulation of albumin uptake by the proximal tubule.

Item Type: Article
Uncontrolled Keywords: ResPubID16886, ClC-5, endocytosis, Cofilin
Subjects: Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
FOR Classification > 0601 Biochemistry and Cell Biology
Depositing User: VUIR
Date Deposited: 25 May 2011 06:21
Last Modified: 19 Jul 2011 02:32
URI: http://vuir.vu.edu.au/id/eprint/2444
DOI: 10.1074/jbc.M307890200
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Citations in Scopus: 59 - View on Scopus

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