Dietary Regulation of Fat Oxidative Gene Expression in Different Skeletal Muscle Fiber Types
McAinch, Andrew and Lee, Jong Sam and Bruce, Clinton R and Tunstall, Rebecca J and Hawley, John A and Cameron-Smith, David (2003) Dietary Regulation of Fat Oxidative Gene Expression in Different Skeletal Muscle Fiber Types. Obesity: a Research Journal, 11 (12). pp. 1471-1479. ISSN 1930-7381Full text for this resource is not available from the Research Repository.
Objective: To determine the effect of a high-fat diet on the expression of genes important for fat oxidation, the protein abundance of the transcription factors peroxisome proliferator-activated receptor (PPAR) isoforms and , and selected enzyme activities in type I and II skeletal muscle. Research Methods and Procedures: Sprague-Dawley rats consumed either a high-fat (HF: 78% energy, n = 8) or high-carbohydrate (64% energy, n = 8) diet for 8 weeks while remaining sedentary. Results: The expression of genes important for fat oxidation tended to increase in both type I (soleus) and type II (extensor digitorum longus) fiber types after an HF dietary intervention. However, the expression of muscle type carnitine palmitoyltransferase I was not increased in extensor digitorum longus. Analysis of the gene expression of both peroxisome proliferator-activated receptor- coactivator and forkhead transcription factor O1 demonstrated no alteration in response to the HF diet. Similarly, PPAR and PPAR protein levels were also not altered by the HF diet. Discussion: An HF diet increased the expression of an array of genes involved in lipid metabolism, with only subtle differences evident in the response within differing skeletal muscle fiber types. Despite changes in gene expression, there were no effects of diet on peroxisome proliferator-activated receptor- coactivator and forkhead transcription factor O1 mRNA and the protein abundance of PPAR and PPAR.
|Uncontrolled Keywords:||ResPubID16919, mRNA, extensor digitorum longus, soleus, Sprague-Dawley, fat metabolism|
|Subjects:||Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
FOR Classification > 1103 Clinical Sciences
SEO Classification > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions)
|Date Deposited:||31 May 2011 05:49|
|Last Modified:||18 Mar 2015 04:31|
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|Citations in Scopus:||23 - View on Scopus|
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