Research Repository

The biocompatibility of titanium cardiovascular devices seeded with autologous blood-derived endothelial progenitor cells: EPC-seeded antithrombotic Ti Implants

Achneck, Hardean E, Jamiolkowski, Ryan M, Jantzen, Alexandra E, Haseltine, Justin M, Lane, Whitney O, Huang, Jessica J, Galinat, Lauren J, Serpe, Michael J, Lin, Fu-Hsiung, Li, Madison, Parikh, Amar, Ma, Liqiao, Chen, Tao, Sileshi, Bantayehu, Milano, Carmelo A, Wallace, Charles S, Stabler, Thomas V, Allen, Jason, Truskey, George A and Lawson, Jeffrey H (2010) The biocompatibility of titanium cardiovascular devices seeded with autologous blood-derived endothelial progenitor cells: EPC-seeded antithrombotic Ti Implants. Biomaterials, 32 (1). pp. 10-18. ISSN 0142-9612

Full text for this resource is not available from the Research Repository.

Abstract

Implantable and extracorporeal cardiovascular devices are commonly made from titanium (Ti) (e.g. Ti-coated Nitinol stents and mechanical circulatory assist devices). Endothelializing the blood-contacting Ti surfaces of these devices would provide them with an antithrombogenic coating that mimics the native lining of blood vessels and the heart. We evaluated the viability and adherence of peripheral blood-derived porcine endothelial progenitor cells (EPCs), seeded onto thin Ti layers on glass slides under static conditions and after exposure to fluid shear stresses. EPCs attached and grew to confluence on Ti in serum-free medium, without preadsorption of proteins. After attachment to Ti for 15 min, less than 5% of the cells detached at a shear stress of 100 dyne / cm2. Confluent monolayers of EPCs on smooth Ti surfaces (Rq of 10 nm), exposed to 15 or 100 dyne / cm2 for 48 h, aligned and elongated in the direction of flow and produced nitric oxide dependent on the level of shear stress. EPC-coated Ti surfaces had dramatically reduced platelet adhesion when compared to uncoated Ti surfaces. These results indicate that peripheral blood-derived EPCs adhere and function normally on Ti surfaces. Therefore EPCs may be used to seed cardiovascular devices prior to implantation to ameliorate platelet activation and thrombus formation.

Item Type: Article
Uncontrolled Keywords: biocompatibility, cell adhesion, progenitor cell, titanium, thrombogenicity, endothelialisation
Subjects: FOR Classification > 0903 Biomedical Engineering
FOR Classification > 1102 Cardiorespiratory Medicine and Haematology
Faculty/School/Research Centre/Department > College of Sports and Exercise Science
Depositing User: Ms Julie Gardner
Date Deposited: 24 Sep 2014 05:05
Last Modified: 26 Sep 2014 07:31
URI: http://vuir.vu.edu.au/id/eprint/25753
DOI: https://doi.org/10.1016/j.biomaterials.2010.08.073
ePrint Statistics: View download statistics for this item
Citations in Scopus: 50 - View on Scopus

Repository staff only

View Item View Item

Search Google Scholar