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Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus

Kong, Lih L and Omar, Abdul and Hair-Bejo, M and Aini, I and Seow, Heng-Fong (2004) Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus. Comparative Immunology, Microbiology and Infectious Diseases, 27 (6). pp. 433-443. ISSN 0147-9571

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Abstract

Specific-pathogen-free (SPF) chickens infected with very virulent (vv) infectious bursal disease virus (IBDV) UPM94/273 developed lower pathogenicity compared to UPM97/61. Sequence analysis indicated that UPM94/273 is an exceptional vvIBDV. In this study, a SYBR® Green I based real-time reverse transcriptase reaction assay was developed to measure viral RNA in the bursae of SPF chickens infected with IBDV. Specificity of the amplified products was confirmed by melting temperature analysis. A linear relationship was observed between the amount of input viral RNA and the threshold values for IBDV-specific product over five log10 dilutions. The viral RNA level following infection with UPM94/273 was significantly higher at day 1 and 2 post-inoculation (p.i.) compared to UPM97/61 infected chickens. However, chickens infected with UPM97/61 had significantly higher numbers of bursal cells undergoing apoptosis compared to UPM94/273 infected chickens. In both groups, the number of apoptotic cells and viral RNA levels peak at day 3 p.i. This study indicates that UPM97/61 and UPM94/273 have different efficiency of replication and percentage of apoptotic cells in bursae during the acute phase of IBDV infection.

Item Type: Article
Uncontrolled Keywords: ResPubID19028, chicken, infectious bursal disease virus, Real-time RT-PCR, Apoptosis
Subjects: Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
FOR Classification > 1107 Immunology
Depositing User: VUIR
Date Deposited: 20 Mar 2012 00:54
Last Modified: 12 Mar 2015 06:41
URI: http://vuir.vu.edu.au/id/eprint/2615
DOI: 10.1016/j.cimid.2004.01.006
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Citations in Scopus: 10 - View on Scopus

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