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HPLC analyses of flavanols and phenolic acids in the fresh young shoots of tea (Camellia sinensis) grown in Australia

Yao, Lihu and Jiang, Yueming and Datta, Nivedita and Singanusong, Riantong and Liu, Xu and Duan, Jun and Raymont, Katherine and Lisle, Alan and Xu, Ying (2004) HPLC analyses of flavanols and phenolic acids in the fresh young shoots of tea (Camellia sinensis) grown in Australia. Food Chemistry, 84 (2). pp. 253-263. ISSN 0308-8146

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Abstract

As part of a 4-year project to study phenolic compounds in tea shoots over the growing seasons and during black tea processing in Australia, an HPLC method was developed and optimised for the identification and quantification of phenolic compounds, mainly flavanols and phenolic acids, in fresh tea shoots. Methanol proved to be the most suitable solvent for extracting the phenolic compounds, compared with chloroform, ethyl acetate and water. Immediate analysis, by HPLC, of the methanol extract showed higher separation efficiency than analyses after being dried and redissolved. This method exhibited good repeatability (CV 3–9%) and recovery rate (88–116%). Epigallocatechin gallate alone constituted up to 115 mg/g, on a dry basis, in the single sample of Australian fresh tea shoots examined. Four catechins (catechin, gallocatechin, epicatechin and epigallocatechin) and six catechin gallates, epigallocatechin gallate, catechin gallate, epicatechin gallate, gallocatechin gallate, epicatechin digallate and epigallocatechin digallate) have been identified and quantified by this HPLC method. In addition, two major tea alkaloids, caffeine and theobromine, have been quantified, while five flavonol glycosides and six phenolic acids, including quinic acids and esters, were identified and quantified.

Item Type: Article
Uncontrolled Keywords: ResPubID18307, alkaloids, flavanols, fresh shoots, HPLC, photodiode array detection, Phenolic acids, tea
Subjects: Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
Depositing User: VUIR
Date Deposited: 20 Mar 2012 01:43
Last Modified: 19 Mar 2015 05:50
URI: http://vuir.vu.edu.au/id/eprint/2678
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Citations in Scopus: 77 - View on Scopus

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