The mycological and molecular study of Hortaea werneckii isolated from blood and splenic abscess
Ng, Kee Peng and Soo-Hoo, T. S and Na, S. L and Tay, S. T and Hamimah, H and Lim, P. C and Chong, Pei Pei and Seow, Heng-Fong and Chavez, A. J and Messer, S. A (2005) The mycological and molecular study of Hortaea werneckii isolated from blood and splenic abscess. Mycopathologia, 159 (4). pp. 495-500. ISSN 0301-486XFull text for this resource is not available from the Research Repository.
Hortaea werneckii is an environmental dematiaceous fungus found in the halophilic environment. It causes tinea nigra. We report the isolation of H. werneckii from blood and splenic abscess of two patients with acute myelomonocytic leukaemia. H. werneckii grew at room temperature but not at 37 °C, it was identified by biochemical tests, growth characteristics and the presence of conspicuous collarette intercalary on dividing yeast cells. The use of specific oligonucleotide primer Hor-F (5′-TGGACACCTTCA TAACTCTTG-3′) and Hor-R (5′-TCACAACGCTTAGAGACGG-3′) confirmed the two isolates were H. werneckii. The sequence for 281 nucleotide of HW299 and HW403 were 99% identical but differed only in one nucleotide. In vitro anti-fungal susceptibility testing showed that the isolates were resistant to amphotericin B and flucytosine.
|Uncontrolled Keywords:||ResPubID19019; acute myeloid leukaemia, dematiaceous fungi, Hortaea werneckii, oligonucleotide primer, tinea nigra|
|Subjects:||FOR Classification > 1199 Other Medical and Health Sciences
Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
|Date Deposited:||28 Mar 2012 05:44|
|Last Modified:||29 Jan 2015 22:12|
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|Citations in Scopus:||5 - View on Scopus|
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