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Antigenicity and immunogenicity of the immunodominant region of hepatitis B surface antigen displayed on bacteriophage T7

Tan, Geok Hun and Yusoff, Khatijah and Seow, Heng Fong and Tan, Wen Siang (2005) Antigenicity and immunogenicity of the immunodominant region of hepatitis B surface antigen displayed on bacteriophage T7. Journal of Medical Virology, 77 (4). pp. 475-480. ISSN 0146-6615

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Abstract

The immunodominant region of hepatitis B virus (HBV) located in the viral small surface antigen (S-HBsAg) elicits virus-neutralizing and protective antibodies. In order to develop an easy and inexpensive method to produce this region without the need for extensive purification, amino acid residues 111–156 of S-HBsAg were fused to the C-terminal end of the 10B capsid protein of T7 phage. Western blotting and ELISA confirmed the expression of the recombinant protein on the surface of the phage particles. The recombinant phage exhibited the antigenic and immunogenic characteristics of HBsAg, illustrating its potential as an immunological reagent and vaccine. J.

Item Type: Article
Uncontrolled Keywords: ResPubID19020, HBV, T7 phage, HBsAg, immunodominant region, phage display, immunology
Subjects: FOR Classification > 1108 Medical Microbiology
Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
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Depositing User: VUIR
Date Deposited: 20 Mar 2012 05:53
Last Modified: 14 Dec 2014 22:40
URI: http://vuir.vu.edu.au/id/eprint/2853
DOI: 10.1002/jmv.20479
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Citations in Scopus: 10 - View on Scopus

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