Differential Regulation of Adiponectin Receptor Gene Expression by Adiponectin and Leptin in Myotubes Derived from Obese and Diabetic Individuals
McAinch, Andrew and Steinberg, Gregory R and Mollica, Janelle and O'Brien, Paul E and Dixon, John B and Macaulay, S Lance and Kemp, Bruce E and Cameron-Smith, David (2006) Differential Regulation of Adiponectin Receptor Gene Expression by Adiponectin and Leptin in Myotubes Derived from Obese and Diabetic Individuals. Obesity: a Research Journal, 14 (11). pp. 1898-1904. ISSN 1930-7381Full text for this resource is not available from the Research Repository.
Objective: This study aimed to investigate the regulation of adiponectin receptors 1 (AdipoR1) and 2 (AdipoR2) gene expression in primary skeletal muscle myotubes, derived from human donors, after exposure to globular adiponectin (gAd) and leptin. Research Methods and Procedures: Four distinct primary cell culture groups were established [ Lean, Obese, Diabetic, Weight Loss (Wt Loss); n = 7 in each] from rectus abdominus muscle biopsies obtained from surgical patients. Differentiated myotube cultures were exposed to gAd (0.1 g/mL) or leptin (2.5 g/mL) for 6 hours. AdipoR1 and AdipoR2 gene expression was measured by real-time polymerase chain reaction analysis. Results: AdipoR1 mRNA expression in skeletal muscle myotubes derived from Lean subjects (p < 0.05) was stimulated 1.8-fold and 2.5-fold with gAd and leptin, respectively. No increase in AdipoR1 gene expression was measured in myotubes derived from Obese, Diabetic, or Wt Loss subjects. AdipoR2 mRNA expression was unaltered after gAd and leptin exposure in all myotube groups. Discussion: Adiponectin and leptin are rapid and potent stimulators of AdipoR1 in myotubes derived from lean healthy individuals. This effect was abolished in myotubes derived from obese, obese diabetic subjects, and obese-prone individuals who had lost significant weight after bariatric surgery. The incapacity of skeletal muscle of obese and diabetic individuals to respond to exogenous adiponectin and leptin may be further suppressed as a result of impaired regulation of the AdipoR1 gene.
|Uncontrolled Keywords:||ResPubID18543, ResPubID14435 mRNA, lipid metabolism, skeletal muscle, cell culture, adiponectin, adult, cells, cultured, diabetes mellitus, female, gene expression regulation, humans, leptin, male, middle aged, muscle fibers, muscle, skeletal, obesity, RNA, messenger, receptors, adiponectin, receptors, cell surface, receptors, leptin, weight loss|
|Subjects:||FOR Classification > 1101 Medical Biochemistry and Metabolomics
Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
|Date Deposited:||19 Aug 2011 01:30|
|Last Modified:||23 Mar 2015 23:42|
|ePrint Statistics:||View download statistics for this item|
|Citations in Scopus:||18 - View on Scopus|
Repository staff only