A sensitive HPLC method for measuring bacterial proteolysis and proteinase activity in UHT milk
Le, T X and Datta, Nivedita and Deeth, H. C (2006) A sensitive HPLC method for measuring bacterial proteolysis and proteinase activity in UHT milk. Food Research International, 39 (7). pp. 823-830. ISSN 0963-9969Full text for this resource is not available from the Research Repository.
A sensitive quantitative reversed-phase HPLC method is described for measuring bacterial proteolysis and proteinase activity in UHT milk. The analysis is performed on a TCA filtrate of the milk. The optimum concentration of TCA was found to be 4%; at lower concentrations, non-precipitated protein blocked the HPLC while higher concentrations yielded lower amounts of peptides. The method showed greater sensitivity and reproducibility than a fluorescamine-based method. Quantification of the HPLC method was achieved by use of an external dipeptide standard or a standard proteinase.
|Uncontrolled Keywords:||ResPubID17940, bacterial proteinase; HPLC, fluorescamine, proteinase activity, proteolysis|
|Subjects:||FOR Classification > 0305 Organic Chemistry
Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
|Date Deposited:||02 May 2012 06:18|
|Last Modified:||03 Dec 2014 03:18|
|ePrint Statistics:||View download statistics for this item|
|Citations in Scopus:||4 - View on Scopus|
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