Isolation and characterization of polymorphic microsatellite loci for the study of paternity and population structure in the little penguin Eudyptula minor
Billing, T. M and Guay, Patrick-Jean and Peucker, A. J and Mulder, R. A (2007) Isolation and characterization of polymorphic microsatellite loci for the study of paternity and population structure in the little penguin Eudyptula minor. Molecular Ecology Notes, 7. pp. 425-427. ISSN 1471-8278Full text for this resource is not available from the Research Repository.
We isolated and characterized eight novel microsatellite loci in the little penguin Eudyptula minor, using nonradioactive polymerase chain reaction-based techniques to screen GA and GAAA repeats from enriched genomic DNA libraries. All eight loci were polymorphic and seven were variable in our main study population. Cross-amplification using a microsatellite primer developed in Spheniscus demersus(African penguin) yielded one additional polymorphic locus. This locus combined with six of the little penguin loci is suitable for paternity assignment in little penguins.
|Uncontrolled Keywords:||ResPubID17193, Eudyptula minor, little penguin, microsatellite, parentage, paternity, population genetics|
|Subjects:||Faculty/School/Research Centre/Department > Institute for Sustainability and Innovation (ISI)
FOR Classification > 0608 Zoology
|Date Deposited:||30 Aug 2011 04:10|
|Last Modified:||24 Jun 2014 06:52|
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|Citations in Scopus:||1 - View on Scopus|
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