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Isolation and characterization of polymorphic microsatellite loci for the study of paternity and population structure in the little penguin Eudyptula minor

Billing, T M and Guay, Patrick-Jean and Peucker, A J and Mulder, R A (2007) Isolation and characterization of polymorphic microsatellite loci for the study of paternity and population structure in the little penguin Eudyptula minor. Molecular Ecology Notes, 7. pp. 425-427. ISSN 1471-8278

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Abstract

We isolated and characterized eight novel microsatellite loci in the little penguin Eudyptula minor, using nonradioactive polymerase chain reaction-based techniques to screen GA and GAAA repeats from enriched genomic DNA libraries. All eight loci were polymorphic and seven were variable in our main study population. Cross-amplification using a microsatellite primer developed in Spheniscus demersus(African penguin) yielded one additional polymorphic locus. This locus combined with six of the little penguin loci is suitable for paternity assignment in little penguins.

Item Type: Article
Uncontrolled Keywords: ResPubID17193, Eudyptula minor, little penguin, microsatellite, parentage, paternity, population genetics
Subjects: Faculty/School/Research Centre/Department > Institute for Sustainability and Innovation (ISI)
FOR Classification > 0608 Zoology
Depositing User: VUIR
Date Deposited: 30 Aug 2011 04:10
Last Modified: 30 Aug 2011 04:10
URI: http://vuir.vu.edu.au/id/eprint/3189
DOI: 10.1111/j.1471-8286.2006.01607.x
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Citations in Scopus: 1 - View on Scopus

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