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Quantitative polarized phase microscopy for birefringence imaging

Dragomir, Nicoleta and Goh, Xiao Ming and Curl, Claire L and Delbridge, Lea M. D and Roberts, Ann (2007) Quantitative polarized phase microscopy for birefringence imaging. Optics Express, 15 (26). pp. 17690-17698. ISSN 1094-4087

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Abstract

A novel application of quantitative phase imaging under linearly polarized light is introduced for studying unstained anisotropic live cells. The method is first validated as a technique for mapping the two-dimensional retardation distribution of a well-characterized optical fiber and is then applied to the characterization of unstained isolated cardiac cells. The experimental retardation measurements are in very good agreement with the established Brace-K hler method, and additionally provide spatially resolved cell birefringence and phase data.

Item Type: Article
Uncontrolled Keywords: ResPubID16372, ResPubID18563. quantitative phase imaging, mapping two-dimensional retardation, optical fibers, cardiac cells, Brace-K hler method, cell birefringence
Subjects: Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
FOR Classification > 0205 Optical Physics
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Depositing User: VUIR
Date Deposited: 07 May 2012 02:57
Last Modified: 12 Feb 2015 00:42
URI: http://vuir.vu.edu.au/id/eprint/3247
DOI: 10.1364/OE.15.017690
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Citations in Scopus: 9 - View on Scopus

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