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Increase in Tumour-Infiltrating Lymphocytes with Regulatory T Cell Immunophenotypes and Reduced S-Chain Expression in Nasopharyngeal Carcinoma Patients

Yip, W and Abdullah, M and Yusoff, S and Seow, Heng-Fong (2009) Increase in Tumour-Infiltrating Lymphocytes with Regulatory T Cell Immunophenotypes and Reduced S-Chain Expression in Nasopharyngeal Carcinoma Patients. Clinical and Experimental Immunology, 155 (3). pp. 412-422. ISSN 0009-9104

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Abstract

The pathological significance of the mechanisms of tumour immune-evasion and/or immunosuppression, such as loss of T cell signalling and increase in regulatory T cells (Tregs), has not been well established in the nasopharyngeal carcinoma (NPC) microenvironment. To evaluate the Treg immunophenotypes in tumour-infiltrating lymphocytes (TILs), we performed a doubleenzymatic immunostaining for detection of forkhead box P3 (FoxP3) and other markers including CD4, CD8, and CD25 on 64 NPC and 36 nonmalignant nasopharyngeal (NP) paraffin-embedded tissues. Expression of CD3z and CD3e was also determined. The prevalence of CD4+FoxP3+ cells in CD4+ T cells and the ratio of FoxP3+/CD8+ were increased significantly in NPC compared with those in NP tissues (P < 0·001 and P = 0·025 respectively). Moreover, the ratio of FoxP3+/CD25+FoxP3- in NPC was significantly lower than that in NP tissues (P = 0·005), suggesting an imbalance favouring activated phenotype of T cells in NPC. A significant negative correlation between the abundance of FoxP3+ and CD25+FoxP3- cells (P < 0·001) was also identified. When histological types of NPC were considered, a lower ratio of FoxP3+/CD25+FoxP3- was found in non-keratinizing and undifferentiated carcinomas. Increased CD4+FoxP3+/CD4+ proportion and FoxP3+/CD8+ ratio were associated with keratinizing squamous cell carcinoma.A reduced expression of CD3z in TILs was found in 20·6% of the NPC tissues but none of the NP tissues. These data provide evidence for the imbalances of Treg and effector T cell phenotypes and down-regulation of signal-transducing molecules in TILs, supporting their role in suppression of immune response and immune evasion of NPC.

Item Type: Article
Uncontrolled Keywords: ResPubID15904. tumours, lymphocytes, regulatory T cell, immunophenotypes, reduced S-chain expression, nasopharyngeal carcinoma patients, nasopharyngeal carcinoma, CD3z, FoxP3, histological type, NPC, Treg, immunosuppression
Subjects: Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
FOR Classification > 1107 Immunology
SEO Classification > 970111 Expanding Knowledge in the Medical and Health Sciences
Depositing User: VUIR
Date Deposited: 16 Aug 2011 05:37
Last Modified: 16 Aug 2011 05:37
URI: http://vuir.vu.edu.au/id/eprint/4073
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