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Leucocyte-rich platelet-rich plasma enhances fibroblast and extracellular matrix activity: Implications in wound healing

Devereaux, Jeannie, Dargahi, Narges, Fraser, Sarah, Nurgali, Kulmira ORCID: 0000-0002-2597-6929, Kiatos, Dimitrios and Apostolopoulos, Vasso ORCID: 0000-0001-6788-2771 (2020) Leucocyte-rich platelet-rich plasma enhances fibroblast and extracellular matrix activity: Implications in wound healing. International Journal of Molecular Sciences, 21 (18). pp. 1-17. ISSN 1661-6596

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Abstract

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Background: Platelet-rich plasma (PRP) is an autologous blood product that contains a high concentration of platelets and leucocytes, which are fundamental fibroblast proliferation agents. L iterature has emerged that offers contradictory findings about leucocytes within PRP. Herein, we elucidated the effects of highly concentrated leucocytes and platelets on human fibroblasts. Methods: Leucocyte-rich, PRP (LR-PRP) and leucocyte-poor, platelet-poor plasma (LP-PPP) were compared to identify their effects on human fibroblasts, including cell proliferation, wound healing and extracellular matrix and adhesion molecule gene expressions. Results: The LR-PRP exhibited 1422.00 ± 317.21 × 103 platelets/µL and 16.36 ± 2.08 × 103 white blood cells/µL whilst the LP-PPP demonstrated lower concentrations of 55.33 ± 10.13 × 103 platelets/µL and 0.8 ± 0.02 × 103 white blood cells/µL. LR-PRP enhanced fibroblast cell proliferation and cell migration, and demonstrated either upregulation or down-regulation gene expression profile of the extracellular matrix and adhesion molecules. Conclusion: LR-PRP has a continuous stimulatory anabolic and ergogenic effect on human fibroblast cells.

Item Type: Article
Uncontrolled Keywords: platelet rich plasma, fibroblasts in vitro, fibroblast proliferation, wound scratch assay, adhesion molecule expression, wound healing
Subjects: Current > FOR Classification > 1107 Immunology
Current > FOR Classification > 1116 Medical Physiology
Current > Division/Research > Institute for Health and Sport
Current > Division/Research > College of Health and Biomedicine
SWORD Depositor: Symplectic Elements
Depositing User: Symplectic Elements
Date Deposited: 30 Sep 2020 02:05
Last Modified: 30 Sep 2020 02:05
URI: http://vuir.vu.edu.au/id/eprint/41168
DOI: https://doi.org/10.3390/ijms21186519
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Citations in Scopus: 0 - View on Scopus

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