Alterations in cardiac dihydropyridine receptors and calcium channel function in mdx mice

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Xiao, xiao-Hui, Woolf, Peter, Watson, Michael, Lu, Sai ORCID: 0000-0002-6368-3721 and Hoey, Andrew (2004) Alterations in cardiac dihydropyridine receptors and calcium channel function in mdx mice. In: XVII World Congress International Society for Heart Research, 7-11 August 2004, Brisbane, Queensland.

Abstract

Duchenne muscular dystrophy (DMD) is a fatal neuromuscular condition affecting approximately one in 3500 live male births resulting from the lack of the myocyte protein dystrophin. The absence of dystrophin in cardiac myocytes is associated with calcium overload which in turn activates calcium-dependent proteolytic enzymes contributing to congestive heart failure, muscle necrosis and fibrosis. To date, the basis for the calcium overload has not been determined. Since L-type calcium channels are a major mediator of calcium influx we determined their potential contribution to the calcium overload. Male muscular dystrophy (mdx) mice and control C57BL10ScSn (C57) mice aged 12– 16 weeks were used in all experiments. In tissue bath studies, isolated contracting left atria from mdx revealed a reduced potency to the dihydropyridine (DHP) agonist BayK8644 and antagonist nifedipine (P < 0.05). Similarly, radioligand binding studies using the DHP antagonist [3H]-PN 200-110 showed a reduced potency (P < 0.05) in isolated membranes, associated with an increased receptor density (P < 0.05). The increased receptor density was supported by RT-PCR experiments revealing increased RNAfor the DHP receptor. Patch clamp studies revealed the presence of a diltiazem sensitive calcium current that showed delayed inactivation in isolated mdx myocytes (P < 0.01). In conclusion, the increased number of DHP binding sites and the delay in L-type current inactivation may both contribute to increased calcium influx and hence calcium overload in the dystrophin deficient mdx cardiac myocytes.

Additional Information

Official link leads to abstract of paper only. Abstract no A40.

Item type Conference or Workshop Item (Paper)
URI http://vuir.vu.edu.au/id/eprint/41264
Identification Number https://doi.org/10.1016/j.yjmcc.2004.05.003
Official URL https://www.sciencedirect.com/science/article/pii/...
Subjects Current > FOR Classification > 0601 Biochemistry and Cell Biology
Current > FOR Classification > 1102 Cardiorespiratory Medicine and Haematology
Current > FOR Classification > 1109 Neurosciences
Current > Division/Research > College of Health and Biomedicine
Keywords DMD, Duchenne muscular dystrophy, cell biology, mice, cardiovascular systems,
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