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Effect of homogenization techniques on reducing the size of microcapsules and the survival of probiotic bacteria therein

Ding, W. K and Shah, Nagendra P (2009) Effect of homogenization techniques on reducing the size of microcapsules and the survival of probiotic bacteria therein. Journal of Food Science, 74 (6). M231-M236. ISSN 0022-1147

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This study investigated 2 different homogenization techniques for reducing the size of calcium alginate beads during the microencapsulation process of 8 probiotic bacteria strains, namely, Lactobacillus rhamnosus, L. salivarius, L. plantarum, L. acidophilus, L. paracasei, Bifidobacterium longum, B. lactis type Bi-04, and B. lactis type Bi-07. Two different homogenization techniques were used, namely, ultra-turrax benchtop homogenizer and MicrofluidicsTM microfluidizer. Various settings on the homogenization equipmentwere studied such as the number of passes, speed (rpm), duration (min), and pressure (psi). The traditional mixing method using a magnetic stirrer was used as a control. The size of microcapsules resulting from the homogenization technique, and the various settings were measured using a light microscope and a stage micrometer. The smallest capsules measuring (31.2 μm) were created with the microfluidizer using 26 passes at 1200 psi for 40 min. The greatest loss in viability of 3.21 log CFU/mL was observed when using the ultra-turrax benchtop homogenizer with a speed of 1300 rpm for 5 min. Overall, both homogenization techniques reduced capsule sizes; however, homogenization settings at high rpmalso greatly reduced the viability of probiotic organisms.

Item Type: Article
Uncontrolled Keywords: ResPubID19147. homogenisation, microencapsulation, probiotics, microcapsules, calcium alginate
Subjects: Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
FOR Classification > 0908 Food Sciences
Depositing User: VUIR
Date Deposited: 17 Oct 2011 00:12
Last Modified: 17 Oct 2011 00:12
DOI: 10.1111/j.1750-3841.2009.01195.x
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Citations in Scopus: 12 - View on Scopus

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