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Structural changes in the epithelium of the small intestine and immune cell infiltration of enteric ganglia following acute mucosal damage and local inflammation

Pontell, Louise and Castelucci, Patricia and Bagyanszki, Maria and Jovic, Tanja and Thacker, Michelle and Nurgali, Kulmira and Bron, Romke and Furness, John B (2009) Structural changes in the epithelium of the small intestine and immune cell infiltration of enteric ganglia following acute mucosal damage and local inflammation. Virchows Arch, 455 (1). pp. 55-65. ISSN 0945-6317

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Abstract

An acute enteritis is commonly followed by intestinal neuromuscular dysfunction, including prolonged hyperexcitability of enteric neurons. Such motility disorders are associated with maintained increases in immune cells adjacent to enteric ganglia and in the mucosa. However, whether the commonly used animal model, trinitrobenzene sulphonate (TNBS)-induced enteritis, causes histological and immune cell changes similar to human enteric neuropathies is not clear. We have made a detailed study of the mucosal damage and repair and immune cell invasion following intralumenal administration of TNBS. Intestines from untreated, sham-operated and TNBS-treated animals were examined at 3 h to 56 days. At 3 h, the mucosal surface was completely ablated, by 6 h an epithelial covering was substantially restored and by 1 day there was full re-epithelialisation. The lumenal epithelium developed from a squamous cell covering to a fully differentiated columnar epithelium with mature villi at about 7 days. Prominent phagocytic activity of enterocytes occurred at 1–7 days. A surge of eosinophils and T lymphocytes associated with the enteric nerve ganglia occurred at 3 h to 3 days. However, elevated immune cell numbers occurred in the lamina propria of the mucosa until 56 days, when eosinophils were still three times normal. We conclude that the disruption of the mucosal surface that causes TNBS-induced ileitis is brief, a little more than 6 h, and causes a transient immune cell surge adjacent to enteric ganglia. This is much briefer than the enteric neuropathy that ensues. Ongoing mucosal inflammatory reaction may contribute to the persistence of enteric neuropathy.

Item Type: Article
Uncontrolled Keywords: ResPubID18848, enteritis, mucosal inflammation, enteric neuropathy, small intestine, irritable bowel syndrome, epithelial repair, immune cells
Subjects: FOR Classification > 0601 Biochemistry and Cell Biology
Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
Depositing User: VUIR
Date Deposited: 27 Apr 2012 02:22
Last Modified: 24 Mar 2013 23:27
URI: http://vuir.vu.edu.au/id/eprint/4534
DOI: 10.1007/s00428-009-0795-x
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Citations in Scopus: 6 - View on Scopus

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