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Akt, AS160, metabolic risk factors and aerobic fitness in middle-aged women

Levinger, Itamar, Howlett, Kirsten, Peake, Jonathan, Garnham, Andrew P, Hare, David L, Jerums, George, Selig, Steve E and Goodman, Craig (2010) Akt, AS160, metabolic risk factors and aerobic fitness in middle-aged women. Exercise Immunology Review, 16. pp. 98-104. ISSN 1077-5552

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Abstract

AIMS: This study investigated the association between the basal (rest) insulin-signaling proteins, Akt, and the Akt substrate AS160, metabolic risk factors, inflammatory markers and aerobic fitness, in middle-aged women with varying numbers of metabolic risk factors for type 2 diabetes. METHODS: Sixteen women (n=16) aged 51.3 +/- 5.1 (mean +/- SD) years provided muscle biopsies and blood samples at rest. In addition, anthropometric characteristics and aerobic power were assessed and the number of metabolic risk factors for each participant was determined (IDF criteria). RESULTS: The mean number of metabolic risk factors was 1.6 +/- 1.2. Total Akt was negatively correlated with IL-1beta (r = -0.45, p = 0.046), IL-6 (r = -0.44, p = 0.052) and TNF-alpha (r = -0.51, p = 0.025). Phosphorylated AS160 was positively correlated with HDL (r = 0.58, p = 0.024) and aerobic fitness (r = 0.51, p = 0.047). Furthermore, a multiple regression analysis revealed that both HDL (t = 2.5, p = 0.032) and VO2peak (t = 2.4, p = 0.037) were better predictors for phosphorylated AS160 than TNF-alpha or IL-6 (p > 0.05). CONCLUSIONS: Elevated inflammatory markers and increased metabolic risk factors may inhibit insulin-signaling protein phosphorylation in middle-aged women, thereby increasing insulin resistance under basal conditions. Furthermore, higher HDL and fitness levels are associated with an increased AS160 phosphorylation, which may in turn reduce insulin resistance.

Item Type: Article
Uncontrolled Keywords: ResPubID20005, insulin signaling, inflammation, cytokines, aerobic fitness
Subjects: FOR Classification > 1101 Medical Biochemistry and Metabolomics
FOR Classification > 1106 Human Movement and Sports Science
SEO Classification > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions)
Faculty/School/Research Centre/Department > Institute of Sport, Exercise and Active Living (ISEAL)
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Depositing User: VUIR
Date Deposited: 20 Jun 2012 04:59
Last Modified: 17 Feb 2015 00:15
URI: http://vuir.vu.edu.au/id/eprint/7481
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Citations in Scopus: 10 - View on Scopus

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