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Effects of Age and Sedentary Lifestyle on Skeletal Muscle NF-κB Signaling in Men

Buford, T and Cooke, Matthew B and Manini, T and Leeuwenburgh, C and Willoughby, D (2010) Effects of Age and Sedentary Lifestyle on Skeletal Muscle NF-κB Signaling in Men. Journals of Gerontology. Series A: Biological Sciences & Medical Sciences , 65A (5). pp. 532-537. ISSN 1079-5006

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Abstract

Nuclear factor kappa B (NF-κB) is a critical signaling molecule of disuse-induced skeletal muscle atrophy. However, few studies have carefully investigated whether similar pathways are modulated with physical activity and age. The present study examined lean mass, maximal force production, and skeletal muscle NF-κB signaling in 41 men categorized as sedentary (OS, N = 13, 63.85 ± 6.59 year), physically active (OA, N = 14, 60.71 ± 5.54 year), or young and sedentary (YS, N = 14, 21.35 ± 3.84 year). Muscle tissue from the vastus lateralis was assayed for messenger RNA (mRNA) expression of the β subunit of IkB kinase (IKKβ), cytosolic protein content of phosphorylated inhibitor of kappa B alpha (pIKBα), and nuclear content of NF-κB subunits p50 and p65. When compared with YS, OS demonstrated age-related muscle atrophy and reduced isokinetic knee extension torque. Physical activity in older individuals preserved maximal isokinetic knee extension torque. OS muscle contained 50% more pIKBα than OA and 61% more pIKBα than YS. Furthermore, nuclear p65 was significantly elevated in OS compared with YS. OS muscle did not differ from either of the other two groups for nuclear p50 or for mRNA expression of IKKβ. These results indicate that skeletal muscle content of nuclear-bound p65 is elevated by age in humans. The elevation in nuclear-bound p65 appears to be at least partially due to significant increases in pIKBα. A sedentary lifestyle appears to play some role in increased IKBα; however, further research is needed to identify downstream effects of this increase.

Item Type: Article
Additional Information:

Online ISSN: 1758-535X

Uncontrolled Keywords: ResPubID21803. sarcopenia, muscle atrophy, muscle strength, proteolysis, age, sedentary lifestyle, skeletal muscle, nuclear-bound p65, NF-κB, nuclear factor kappa B, IKBα
Subjects: Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
FOR Classification > 1101 Medical Biochemistry and Metabolomics
FOR Classification > 1106 Human Movement and Sports Science
Depositing User: VUIR
Date Deposited: 17 Nov 2011 04:03
Last Modified: 03 Dec 2014 01:49
URI: http://vuir.vu.edu.au/id/eprint/7540
DOI: 10.1093/gerona/glp196
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Citations in Scopus: 29 - View on Scopus

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