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Effects of Eccentric Treadmill Exercise on Inflammatory Gene Expression in Human Skeletal Muscle

Buford, T, Cooke, Matthew B, Shelmadine, B, Hudson, G, Redd, L and Willoughby, D (2009) Effects of Eccentric Treadmill Exercise on Inflammatory Gene Expression in Human Skeletal Muscle. Applied Physiology, Nutrition and Metabolism, 34 (4). pp. 745-753. ISSN 1715-5312

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Abstract

The present study examined the skeletal muscle expression of several genes related to the inflammatory process before and after a bout of downhill running. Twenty-nine males between the ages of 18 and 35 years performed a 45-min downhill (–17.5%) treadmill protocol at 60% of maximal oxygen consumption. Venous bloods samples and muscle biopsy samples from the vastus lateralis were donated prior to and at 3-h and 24-h postexercise, along with ratings of perceived muscle soreness. Serum creatine kinase (CK) was determined, as was skeletal muscle gene expression of interleukin (IL)-6, IL-8, IL-12 (p35), tumor necrosis factor-alpha (TNF-a), IL-1b, cyclooxygenase 2 (COX2), and nuclear factor kappa B (NFkB) (p105/p50). Gene expression was analyzed using RT-PCR and compared with a standard housekeeping gene (b-actin). Data were analyzed for statistical differences using multivariate analysis of variance with univariate follow-up. In addition, Pearson correlations were conducted to determine if any significant relationship exists between any of these transcripts and both CK and muscle soreness. Significant (p < 0.05) up-regulations in IL-6, IL-8, and COX2 mRNA expression were observed compared with baseline, whereas no significant changes for IL-12, IL-1b, TNF-a, or NFkB were noted. Significant increases in IL-6 mRNA were observed at 3 h (p < 0.001) and 24 h (p = 0.043), whereas significant increases in IL-8 (p = 0.001) and COX2 (p = 0.046) mRNA were observed at 3-h postexercise. In addition, muscle soreness was significantly correlated with IL-8 at 24 h (r = –0.370; p = 0.048), whereas CK was significantly related to NFkB at baseline (r = –0.460; p = 0.012). These data indicate that increases in the mRNA expression of IL-6, IL-8, and COX2 occur in the vastus lateralis as a result of damaging eccentric exercise in young, recreationally trained males. Further, it appears that IL-8 transcription may play some role in inhibiting postexercise muscle soreness, possibly through regulation of angiogenesis.

Item Type: Article
Uncontrolled Keywords: ResPubID21813. cytokines, eccentric exercise, gene expression, inflammation, skeletal muscle damage, expression génique, lésion musculaire, pliométrique
Subjects: Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
FOR Classification > 1106 Human Movement and Sports Science
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Depositing User: VUIR
Date Deposited: 02 Feb 2012 03:57
Last Modified: 03 Dec 2014 01:51
URI: http://vuir.vu.edu.au/id/eprint/7546
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Citations in Scopus: 51 - View on Scopus

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