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The use of adipose tissue-conditioned media to demonstrate the differential effects of fat depots on insulin-stimulated glucose uptake in a skeletal muscle cell line

Lam, Yan and Janovska, Alena and McAinch, Andrew and Belobrajdic, Damien and Hatzinikolas, George and Game, Philip and Wittert, Gary (2011) The use of adipose tissue-conditioned media to demonstrate the differential effects of fat depots on insulin-stimulated glucose uptake in a skeletal muscle cell line. Obesity Research and Clinical Practice, 5 (1). e43-e54. ISSN 1871-403X

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Abstract

We aimed to study the depot-specific effect of adipose tissue on insulin sensitivity of skeletal muscle in vitro. Adipose tissue-conditioned medium (CM) was generated from visceral and subcutaneous fat from obese subjects. CM from visceral as compared to subcutaneous fat had higher concentrations of interleukin (IL)-6 (15-fold; P < 0.05) and IL-8 (8-fold; P < 0.05). CM from visceral fat (1:128 dilution) reduced insulin-stimulated glucose uptake in L6 myotubes by 19% (P < 0.05), an effect mediated by a nuclear factor kappa B (NFκB)/mammalian target of rapamycin complex 1 (mTORC1)-dependent pathway and partially reversed by neutralizing IL-6. IL-6 at a concentration comparable to that in CM from visceral fat reduced insulin-stimulated glucose uptake by 53% (P < 0.05), an effect abolished by inhibiting NFκB or mTORC1. We demonstrated the utility of the CM-myotube system and identified IL-6 as a major cytokine mediating visceral fat-induced muscle insulin resistance.

Item Type: Article
Uncontrolled Keywords: ResPubID22868, adipokines, skeletal muscle, insulin resistance
Subjects: Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
FOR Classification > 1116 Medical Physiology
SEO Classification > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions)
Depositing User: VUIR
Date Deposited: 27 Jun 2012 03:30
Last Modified: 05 Jun 2013 23:49
URI: http://vuir.vu.edu.au/id/eprint/7641
DOI: 10.1016/j.orcp.2010.12.002
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Citations in Scopus: 4 - View on Scopus

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