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Nucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptake-encoding region

Blatch, Gregory L, Scholle, R and Woods, D (1990) Nucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptake-encoding region. Gene, 95 (1). pp. 17-23. ISSN 0378-1119

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Abstract

The nucleotide sequence of the Vibrio alginolyticus sucrose uptake-encoding region was determined, and contained two genes, scrA and scrK. The scrA gene encodes an enzyme IISucrose (EIIScr) protein of the phosphoenolpyruvate dependent phosphotransferase system and the scrK gene encodes a fructokinase. The deduced amino acid (aa)_sequence for the V. alginolyticus EIIScr protein was homologous with the EIIScr protein from Streptococcus mutants, Salmonella typhimurium (pUR400 system) and Bacillus subtilis. The deduced aa sequence for the V. alginolyticus fructokinase was homologous with the Escherichia coli enzymes, 6-phosphofructokinase (isoenzyme 2) and ribokinase. Transposon phoA mutagenesis experiments indicated that the EIIScr protein was a membrane-bound protein with a region that extended into the periplasm.

Item Type: Article
Uncontrolled Keywords: ResPubID22242. Bacillus subtilis, Streptococcus mutants, sucrase, fructokinase, transposon, recombinant DNA
Subjects: Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
FOR Classification > 0604 Genetics
Depositing User: VUIR
Date Deposited: 12 Dec 2011 00:56
Last Modified: 15 Dec 2014 22:38
URI: http://vuir.vu.edu.au/id/eprint/7731
DOI: https://doi.org/10.1016/0378-1119(90)90408-J
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Citations in Scopus: 27 - View on Scopus

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