Research Repository

GeneClip™ U1 Hairpin Cloning Systems for expression of short hairpin RNAs in vivo

Yeager, Thomas and Bailey, C and Vidugiriene, J and Almond, B and Storts, D (2005) GeneClip™ U1 Hairpin Cloning Systems for expression of short hairpin RNAs in vivo. Promega Notes, 89 (1). pp. 21-24. ISSN n/a

Full text for this resource is not available from the Research Repository.

Abstract

The GeneClip™ U1 Hairpin Cloning Systems enable cloning and expression of short hairpin RNAs (shRNAs) for RNA interference (RNAi) experiments. The GeneClip™ Systems contain linearized vectors that facilitate cloning of complementary oligonucleotides downstream of a U1 promoter. Upon transfection with pGeneClip™ Vectors containing these cloned sequences, shRNA is produced in the cell, leading to reduction of target gene expression through RNAi. In this article we illustrate use of the GeneClip™ Systems to suppress Renilla luciferase expression in a range of cell types and to suppress p53 expression in both a transient and stable manner.

Item Type: Article
Uncontrolled Keywords: ResPubID22570. hairpin RNAs, GeneClip™ Hairpin Cloning Systems, RNA, genes
Subjects: FOR Classification > 0604 Genetics
Faculty/School/Research Centre/Department > School of Engineering and Science
Depositing User: VUIR
Date Deposited: 19 Jan 2012 01:37
Last Modified: 19 Jan 2012 01:37
URI: http://vuir.vu.edu.au/id/eprint/7824
ePrint Statistics: View download statistics for this item

Repository staff only

View Item View Item

Search Google Scholar