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High-intensity exercise decreases muscle buffer capacity via a decrease in protein buffering in human skeletal muscle

Bishop, David and Edge, Johann and Mendez-Villanueva, A and Thomas, Claire and Schneiker, K (2009) High-intensity exercise decreases muscle buffer capacity via a decrease in protein buffering in human skeletal muscle. Pflugers Archiv European Journal of Physiology, 458 (5). pp. 929-936. ISSN 0031-6768

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Abstract

We have previously reported an acute decrease in muscle buffer capacity (βmin vitro) following high-intensity exercise. The aim of this study was to identify which muscle buffers are affected by acute exercise and the effects of exercise type and a training intervention on these changes. Whole muscle and non-protein βmin vitro were measured in male endurance athletes (VO2max = 59.8 ± 5.8 mL kg−1 min−1), and before and after training in male, team-sport athletes (VO2max = 55.6 ± 5.5 mL kg−1 min−1). Biopsies were obtained at rest and immediately after either time-to-fatigue at 120% VO2max (endurance athletes) or repeated sprints (team-sport athletes). High-intensity exercise was associated with a significant decrease in βmin vitro in endurance-trained males (146 ± 9 to 138 ± 7 mmol H+·kg d.w.−1·pH−1), and in male team-sport athletes both before (139 ± 9 to 131 ± 7 mmol H+·kg d.w.−1·pH−1) and after training (152 ± 11 to 142 ± 9 mmol H+·kg d.w.−1·pH−1). There were no acute changes in non-protein buffering capacity. There was a significant increase in βmin vitro following training, but this did not alter the post-exercise decrease in βmin vitro. In conclusion, high-intensity exercise decreased βmin vitro independent of exercise type or an interval-training intervention; this was largely explained by a decrease in protein buffering. These findings have important implications when examining training-induced changes in βmin vitro. Resting and post-exercise muscle samples cannot be used interchangeably to determine βmin vitro, and researchers must ensure that post-training measurements of βmin vitro are not influenced by an acute decrease caused by the final training bout.

Item Type: Article
Uncontrolled Keywords: ResPubID21988. acidosis, activity, fatigue, buffer capacity, lactate transport, hydrogen ions, blood lactate, intense exercise
Subjects: FOR Classification > 1106 Human Movement and Sports Science
Faculty/School/Research Centre/Department > Institute of Sport, Exercise and Active Living (ISEAL)
Depositing User: VUIR
Date Deposited: 29 May 2012 04:05
Last Modified: 05 Jan 2014 22:58
URI: http://vuir.vu.edu.au/id/eprint/7954
DOI: 10.1007/s00424-009-0673-z
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Citations in Scopus: 9 - View on Scopus

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