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Differential attenuation of AMPK activation during acute exercise following exercise training or AICAR treatment

McConell, Glenn K and Manimmanakorn, A and Lee-Young, Robert S and Kemp, Bruce E and Linden, Kelly C and Wadley, Glenn D (2008) Differential attenuation of AMPK activation during acute exercise following exercise training or AICAR treatment. Journal of Applied Physiology, 105 (5). pp. 1422-1427. ISSN 8750-7587

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Abstract

Short-term exercise training in humans attenuates AMP-activated protein kinase (AMPK) activation during subsequent exercise conducted at the same absolute workload. Short-term 5-aminoimidazole-4-carboxyamide- ribonucleoside (AICAR) administration in rats mimics exercise training on skeletal muscle in terms of increasing insulin sensitivity, mitochondrial enzymes, and GLUT4 content, but it is not known whether these adaptations are accompanied by reduced AMPK activation during subsequent exercise. We compared the effect of 10 days of treadmill training (60 min/day) with 10 days of AICAR administration (0.5 mg/g body weight ip) on subsequent AMPK activation during 45 min of treadmill exercise in male Sprague-Dawley rats. Compared with nonexercised control rats, acute exercise significantly (P < 0.05) increased AMPKα Thr172 phosphorylation (p-AMPKα; 1.6-fold) and ACCβ Ser218 phosphorylation (p-ACCβ; 4.9-fold) in the soleus and p-ACCβ 2.2-fold in the extensor digitorum longus. Ten days of exercise training abolished the increase in soleus p-AMPKα and attenuated the increase in p-ACCβ (nonsignificant 2-fold increase). Ten days of AICAR administration also attenuated the exercise-induced increases in AMPK signaling in the soleus although not as effectively as 10 days of exercise training (nonsignificant 1.3-fold increase in p-AMPKα; significant 3-fold increase in p-ACCβ). The increase in skeletal muscle 2-deoxyglucose uptake during exercise was greater after either 10 days of exercise training or AICAR administration. In conclusion, 10 days of AICAR administration substantially mimics the effect of 10 days training on attenuating skeletal muscle AMPK activation in response to subsequent exercise.

Item Type: Article
Additional Information:

Online ISSN: 1522-1601

Uncontrolled Keywords: ResPubID22025. glucose uptake, glycogen, skeletal muscle, acetyl-CoA carboxylase, intense exercise, diet
Subjects: FOR Classification > 0601 Biochemistry and Cell Biology
FOR Classification > 0606 Physiology
FOR Classification > 1106 Human Movement and Sports Science
Faculty/School/Research Centre/Department > Institute of Sport, Exercise and Active Living (ISEAL)
Depositing User: VUIR
Date Deposited: 26 Apr 2012 23:17
Last Modified: 18 Mar 2015 04:50
URI: http://vuir.vu.edu.au/id/eprint/7988
DOI: 10.​1152/​japplphysiol.​01371.​2007
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Citations in Scopus: 13 - View on Scopus

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