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Effects of high-intensity training on MCT1, MCT4, and NBC expressions in rat skeletal muscles: Influence of chronic metabolic alkalosis

Thomas, Claire, Bishop, David, Moore-Morris, T and Mercier, Jacques (2007) Effects of high-intensity training on MCT1, MCT4, and NBC expressions in rat skeletal muscles: Influence of chronic metabolic alkalosis. American Journal of Physiology - Endocrinology and Metabolism, 293 (4). E916-E922. ISSN 0193-1849 (print) 1522-1555 (online)

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Abstract

This study investigated the effects of high-intensity training, with or without induced metabolic alkalosis, on lactate transporter (MCT1 and MCT4) and sodium bicarbonate cotransporter (NBC) content in rat skeletal muscles. Male Wistar rats performed high-intensity training on a treadmill 5 times/wk for 5 wk, receiving either sodium bicarbonate (ALK-T) or a placebo (PLA-T) prior to each training session, and were compared with a group of control rats (CON). MCT1, MCT4, and NBC content was measured by Western blotting in soleus and extensor digitorum longus (EDL) skeletal muscles. Citrate synthase (CS) and phosphofructokinase (PFK) activities and muscle buffer capacity (betam) were also evaluated. Following training, CS and PFK activities were significantly higher in the soleus only (P < 0.05), whereas betam was significantly higher in both soleus and EDL (P < 0.05). MCT1 (PLA-T: 30%; ALK-T: 23%) and NBC contents (PLA-T: 85%; ALK-T: 60%) increased significantly only in the soleus following training (P < 0.01). MCT4 content in the soleus was significantly greater in ALK-T (115%) but not PLA-T compared with CON. There was no significant change in protein content in the EDL. Finally, NBC content was related only to MCT1 content in soleus (r = 0.50, P < 0.01). In conclusion, these results suggest that MCT1, MCT4, and NBC undergo fiber-specific adaptive changes in response to high-intensity training and that induced alkalosis has a positive effect on training-induced changes in MCT4 content. The correlation between MCT1 and NBC expression suggests that lactate transport may be facilitated by NBC in oxidative skeletal muscle, which may in turn favor better muscle pH regulation.

Item Type: Article
Uncontrolled Keywords: ResPubID22066. intense exercise, MCT1, MCT4, NBC, rats, skeletal muscles, metabolic alkalosis, metabolism, monocarboxylate transporter, sodium bicarbonate transporter, lactate transporter, muscle pH regulation
Subjects: FOR Classification > 1101 Medical Biochemistry and Metabolomics
FOR Classification > 1106 Human Movement and Sports Science
Faculty/School/Research Centre/Department > Institute of Sport, Exercise and Active Living (ISEAL)
Depositing User: VUIR
Date Deposited: 04 Apr 2012 06:35
Last Modified: 06 Jan 2014 01:06
URI: http://vuir.vu.edu.au/id/eprint/8025
DOI: https://doi.org/10.​1152/​ajpendo.​00164.​2007
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Citations in Scopus: 36 - View on Scopus

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