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Effect of nitric oxide synthase inhibition on mitochondrial biogenesis in rat skeletal muscle

Wadley, G. D and McConell, Glenn ORCID: 0000-0002-8572-9065 (2007) Effect of nitric oxide synthase inhibition on mitochondrial biogenesis in rat skeletal muscle. Journal of Applied Physiology, 102 (1). pp. 314-320. ISSN 8750-7587

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Abstract

The purpose of this study was to determine whether nitric oxide synthase (NOS) inhibition decreased basal and exercise-induced skeletal muscle mitochondrial biogenesis. Male Sprague-Dawley rats were assigned to one of four treatment groups: NOS inhibitor NG-nitro-L-arginine methyl ester (L-NAME, ingested for 2 days in drinking water, 1 mg/ml) followed by acute exercise, no L-NAME ingestion and acute exercise, rest plus L-NAME, and rest without L-NAME. The exercised rats ran on a treadmill for 53 ± 2 min and were then killed 4 h later. NOS inhibition significantly (P < 0.05; main effect) decreased basal peroxisome proliferator-activated receptor-γ coactivator 1beta (PGC-1beta) mRNA levels and tended (P = 0.08) to decrease mtTFA mRNA levels in the soleus, but not the extensor digitorum longus (EDL) muscle. This coincided with significantly reduced basal levels of cytochrome c oxidase (COX) I and COX IV mRNA, COX IV protein and COX enzyme activity following NOS inhibition in the soleus, but not the EDL muscle. NOS inhibition had no effect on citrate synthase or beta-hydroxyacyl CoA dehydrogenase activity, or cytochrome c protein abundance in the soleus or EDL. NOS inhibition did not reduce the exercise-induced increase in peroxisome proliferator-activated receptor-γ coactivator 1α (PGC-1α) mRNA in the soleus or EDL. In conclusion, inhibition of NOS appears to decrease some aspects of the mitochondrial respiratory chain in the soleus under basal conditions, but does not attenuate exercise-induced mitochondrial biogenesis in the soleus or in the EDL.

Item Type: Article
Additional Information:

Online ISSN: 1522-1601

Uncontrolled Keywords: ResPubID22100. nitric oxide synthase, mitochondrial biogenesis, NOS isoform, basal, rats, skeletal muscles
Subjects: Current > FOR Classification > 0601 Biochemistry and Cell Biology
Historical > Faculty/School/Research Centre/Department > Institute of Sport, Exercise and Active Living (ISEAL)
Depositing User: VUIR
Date Deposited: 11 Apr 2012 00:07
Last Modified: 02 May 2019 04:47
URI: http://vuir.vu.edu.au/id/eprint/8055
DOI: https://doi.org/10.​1152/​japplphysiol.​00549.​2006
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Citations in Scopus: 49 - View on Scopus

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