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Leukemia inhibitory factor promotes Hsp90 association with STAT3 in mouse embryonic stem cells

Setati, Mokgadi M and Prinsloo, Earl and Longshaw, Victoria M and Murray, Patricia A and Edgar, David H and Blatch, Gregory L (2010) Leukemia inhibitory factor promotes Hsp90 association with STAT3 in mouse embryonic stem cells. IUBMB Life, 62 (1). pp. 61-66. ISSN 1521-6543 (print) 1521-6551 (online)

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Abstract

Self-renewal of in vitro cultured mouse embryonic stem (mES) cells is dependent on the presence of leukemia inhibitory factor (LIF). LIF induces overexpression and tyrosine phosphorylation of STAT3 (signal transducer and activator of transcription 3) and its subsequent nuclear translocation. The molecular chaperone heat shock protein 90 (Hsp90) is involved in the activation and maturation of a wide variety of substrate proteins. We investigated the effect of LIF withdrawal on the protein expression levels of STAT3 and Hsp90 and on the interactions between STAT3 and Hsp90. Taken together the data presented here suggest that LIF promotes the interaction of Hsp90 with STAT3 during self-renewal, indicating a potentially pivotal role for Hsp90 in the LIF-based maintenance of self-renewal of mouse embryonic stem cells.

Item Type: Article
Uncontrolled Keywords: ResPubID22187, mouse, embryonic stem cells, leukemia inhibitory factor, signal transducer and activator of Transcription 3, heat shock protein
Subjects: Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
FOR Classification > 1101 Medical Biochemistry and Metabolomics
Depositing User: VUIR
Date Deposited: 12 Jul 2012 04:49
Last Modified: 23 Mar 2015 02:48
URI: http://vuir.vu.edu.au/id/eprint/8133
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Citations in Scopus: 7 - View on Scopus

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