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Stress protein responses in South African freshwater invertebrates exposed to detergent surfactant linear alkylbenzene sulfonate (LAS)

Gordon, Andrew K and Blatch, Gregory L and Daniel, Sheryl and Muller, Wilhelmine J (2008) Stress protein responses in South African freshwater invertebrates exposed to detergent surfactant linear alkylbenzene sulfonate (LAS). Water, Air, and Soil Pollution, 193 (1-4). pp. 123-130. ISSN 0049-6979

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Abstract

Stress responses can be measured at various levels of biological organization, from sub-organism through to ecosystem level. This study aimed to investigate stress protein induction as a sub-organism level stress response in two South African freshwater aquatic macroinvertebrates exposed to detergent linear alkylbenzene sulfonate (LAS). Shrimp Caridina nilotica and limpet Burnupia stenochorias were exposed to a range of LAS concentrations (0, 1, 1.8, 3.2, 6.5 and 12.7 mg/L and 0, 0.6, 1.2, 1.6 and 3.2 mg/L respectively) for 96 h. Surviving organisms were prepared for sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blot analysis. In C. nilotica there appeared to be induction of a putative ≈ 70 kDa protein at 12.7 mg/L LAS, and induction of putative ≈45 and ≈40 kDa proteins at concentrations of 6.5 mg/L LAS and above. However, only an Hsp70 protein was detected with anti-Hsp72/Hsc73 at 12.7 mg/L LAS. No protein induction was observed in exposed B. stenochorias, however an Hsp40 protein was detected with anti-Hsp40 in exposed and unexposed limpets.

Item Type: Article
Additional Information:

Online ISSN: 1573-2932

Uncontrolled Keywords: ResPubID22201. heat shock proteins, Hsp40, Hsp70, laundry detergent, water quality management
Subjects: Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
FOR Classification > 0502 Environmental Science and Management
FOR Classification > 0601 Biochemistry and Cell Biology
FOR Classification > 0602 Ecology
Depositing User: VUIR
Date Deposited: 23 Apr 2012 07:23
Last Modified: 04 Feb 2015 02:49
URI: http://vuir.vu.edu.au/id/eprint/8147
DOI: 10.1007/s11270-008-9676-1
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Citations in Scopus: 0 - View on Scopus

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