Stress-inducible, murine protein mSTI1: Characterization of binding domains for heat shock proteins and in vitro phosphorylation by different kinases

Full text for this resource is not available from the Research Repository.

Lässle, M, Blatch, Gregory ORCID: 0000-0003-0778-8577, Kundra, V, Takatori, T and Zetter, B (1997) Stress-inducible, murine protein mSTI1: Characterization of binding domains for heat shock proteins and in vitro phosphorylation by different kinases. Tthe Journal of Biological Chemistry, 272 (3). pp. 1876-1884. ISSN 0021-9258

Abstract

We have recently isolated the cDNA for the murine homologue of the stress-inducible phosphoprotein STI1 (also known as IEF SSP 3521 or p60). STI1 was previously shown to be 2-fold up-regulated in MRC-5 fibroblasts upon viral transformation and to exist in a macromolecular complex with heat shock proteins of the HSP 70 and 90 families. By peptide-sequencing we have identified the two heat shock proteins that bind to murine STI1 (mSTI1) as HSC 70 and HSP 84/86. We describe two separate binding regions within mSTI1 for the two heat shock proteins. In the presence of cell extracts, the N-terminal region of mSTI1 binds preferentially to HSC 70, whereas the C-terminal portion of the molecule promotes the binding of HSP 84/86. Heat treatment caused a strong induction of mSTI1 message without affecting the steady-state level of the protein significantly. In addition, heat treatment led to changes in the isoformcomposition of mSTI1. pp70s6k, pp90rsk, and mitogenactivated protein kinase-activated protein kinase 2 were tested as possible STI1 kinases in vitro using recombinant mSTI1 as a substrate: only pp90rsk was able to phosphorylate recombinant mSTI1. In vitro kinase assays using casein kinase II suggest serine 189 to be a likely phosphorylation site in mSTI1.

Dimensions Badge

Altmetric Badge

Item type Article
URI https://vuir.vu.edu.au/id/eprint/8182
DOI 10.1074/jbc.272.3.1876
Official URL http://dx.doi.org/10.1074/jbc.272.3.1876
Subjects Historical > Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
Historical > FOR Classification > 0601 Biochemistry and Cell Biology
Historical > FOR Classification > 0604 Genetics
Keywords ResPubID22237. mice, murine proteins, mSTI1, molecular binding, heat treatment, cDNA, DNA, in vitro phosphorylation, kinases
Citations in Scopus 143 - View on Scopus
Download/View statistics View download statistics for this item

Search Google Scholar

Repository staff login