Survival, Acid and Bile Tolerance, and Surface Hydrophobicity of Microencapsulated B. animalis ssp. lactis Bb12 during Storage at Room Temperature

Full text for this resource is not available from the Research Repository.

Dianawati, Dianawati and Shah, Nagendra P (2011) Survival, Acid and Bile Tolerance, and Surface Hydrophobicity of Microencapsulated B. animalis ssp. lactis Bb12 during Storage at Room Temperature. Journal of Food Science, 76 (9). M592-M599. ISSN 0022-1147

Abstract

Survival, acid and bile tolerance, and surface hydrophobicity of microencapsulated Bifidobacterium animalis ssp. lactis Bb12 were studied during storage at room temperature (25 ◦C) at low water activity (0.07, 0.1, and 0.2). Two types of alginate-based systems were prepared with and without mannitol as microencapsulant of B. animalis ssp. lactis Bb12. Formation of gel beads containing cells was achieved by dropping each emulsion into CaCl(2) solution; then, the beads were freeze dried. Survival, acid tolerance during 2-h exposure in de Man, Rogosa, Sharpe (MRS) broth at pH 2.0, bile tolerance during 8-h exposure in MRS broth containing taurocholic acid at pH 5.8, and retention of surface hydrophobicity were determined after freeze drying and during storage. The result showed that neither alginate nor alginate-mannitol formulation was effective in protecting B. animalis ssp. lactis Bb12 during freezing and freeze drying. The viability in alginate-mannitol and alginate formulations after freeze drying was 6.61 and 6.34 log CFU/g, respectively. Storage at low a(w) improved survival, acid tolerance, bile tolerance, and surface hydrophobicity retention of microencapsulated B. animalis ssp. lactis Bb12 when compared with controlled storage in an aluminum foil (with a(w) of 0.38 and 0.40 for alginate-mannitol and alginate formulations, respectively). Alginate mannitol was more effective than the alginate system during a short period of storage, but its effectiveness decreased during a long period of storage (80% survival at 10 wk). Nevertheless, storage of microencapsulated B. animalis ssp. lactis Bb12 in an aluminum foil without a(w) adjustment during 10 wk at room temperature was not effective (survival was 64% to 65%).

Dimensions Badge

Altmetric Badge

Additional Information

Nov-Dec issue

Item type Article
URI https://vuir.vu.edu.au/id/eprint/10377
DOI https://doi.org/10.1111/j.1750-3841.2011.02422.x
Official URL http://dx.doi.org/10.1111/j.1750-3841.2011.02422.x
Subjects Historical > Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
Historical > FOR Classification > 0908 Food Sciences
Keywords ResPubID24893, alginate-based microencapsulation, fidobacteria, long-term storage, probiotic bacteria, acid tolerance, microencapsulated bacteria, surface hydrophobicity, sodium alginate
Citations in Scopus 28 - View on Scopus
Download/View statistics View download statistics for this item

Search Google Scholar

Repository staff login