MHC-based fiber type and E-C coupling characteristics in mechanically skinned muscle fibers of the rat

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Goodman, Craig, Patterson, Michael and Stephenson, Gabriela (2003) MHC-based fiber type and E-C coupling characteristics in mechanically skinned muscle fibers of the rat. American journal of physiology: cell physiology, 284. c1448-c1459. ISSN 03636143

Abstract

MHC-based fiber type and E-C coupling characteristics in mechanically skinned muscle fibers of the rat. Am J Physiol Cell Physiol 284: C1448–C1459, 2003. First published January 29, 2003; 10.1152/ajpcell.00569.2002.—In this study, we investigated whether the previously established differences between fast- and slow-twitch single skeletal muscle fibers of the rat, in terms of myosin heavy chain (MHC) isoform composition and contractile function, are also detectable in excitation-contraction (E-C) coupling. We compared the contractile responsiveness of electrophoretically typed, mechanically skinned single fibers from the soleus (Sol), the extensor digitorum longus (EDL), and the white region of the sternomastoid (SM) muscle to t-system depolarization- induced activation. The quantitative parameters assessed were the amplitude of the maximum depolarizationinduced force response (DIFRmax; normalized to the maximum Ca2-activated force in that fiber) and the number of responses elicited until the force declined by 75% of DIFRmax (R-D75%). The mean DIFRmax values for type IIB EDL and type IIB SM fibers were not statistically different, and both were greater than the mean DIFRmax for type I Sol fibers. The mean R-D75% for type IIB EDL fibers was greater than that for type I Sol fibers as well as type IIB SM fibers. These data suggest that E-C coupling characteristics of mechanically skinned rat single muscle fibers are related to MHCbased fiber type and the muscle of origin.

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Item type Article
URI https://vuir.vu.edu.au/id/eprint/1054
DOI https://doi.org/10.1152/ajpcell.00569.2002
Official URL http://ajpcell.physiology.org/cgi/content/abstract...
Funders http://purl.org/au-research/grants/nhmrc/256602
Subjects Historical > RFCD Classification > 210000 Science-General
Historical > RFCD Classification > 240000 Physical Sciences
Historical > Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
Keywords excitation-contraction coupling, myosin heavy chain, single muscle fiber, hybrid fiber
Citations in Scopus 16 - View on Scopus
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