Jang, Ki-Hyo (1998) Identification and characterisation of the cell surface and enzymatic barriers to plasmid transformation in Corynebacterium glutamicum and related species. PhD thesis, Victoria University of Technology.

Abstract

Non-pathogenic corynebacteria including Corynebacterium glutamicum, Brevibacterium flavum, and B. lactofermentum have been used traditionally for industrial production of amino acids. Recently, these strains have been also studied using molecular biology or recombinant DNA approaches. However, a major early limitation in the application of this technology was poor transformation efficiency due to the presence of barriers which prevented entry or survival of incoming DNA. These barriers are both physical (cell surface structures) and enzymatic (restriction and modification) barriers. The research described in this thesis involved investigating the nature of both the physical and enzymatic barriers, includings: (i) determining the effect of the presence of glycine and/or isonicotinic acid hydrazide (INH) and resulting lipid profiles (mycotic acids and fatty acids) of several strains of corynebacteria. (ii) examining the enzymatic barriers to transformation of these corynebacteria with specific reference to methyltransferase (MTase) activity and the sites of methylation.

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