Isolation of low ethanol producing yeast strains using adaptive evolution

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Kutyna, Dariusz Roman (2008) Isolation of low ethanol producing yeast strains using adaptive evolution. PhD thesis, Victoria University.

Abstract

This thesis describes the application of a non-genetic engineering approach, adaptive evolution, to generate variants of Saccharomyces cerevisiae that produce reduced levels of ethanol relative to the strains they were derived from. Sub-lethal concentrations of sulfite, which sequesters acetaldehyde, thereby limiting ethanol production, was used as a selection pressure. However, removal of acetaldehyde not only limits ethanol production, it also compromises redox balance by preventing the fermentative oxidation of NADH. To compensate for this, the cell regenerates NAD by producing increased amounts of glycerol; glycerol production is an NADH-driven reductive process. It was demonstrated in this thesis that culturing yeast in presence of sulfite for a number of generation, results in the generation of genetically stable variants with increased glycerol and reduced ethanol production.

Item type Thesis (PhD thesis)
URI https://vuir.vu.edu.au/id/eprint/15484
Subjects Historical > FOR Classification > 0908 Food Sciences
Historical > FOR Classification > 0601 Biochemistry and Cell Biology
Historical > Faculty/School/Research Centre/Department > School of Engineering and Science
Keywords Fermentation, Molecular genetics, Saccharomyces cerevisiae, ethanol, sulfite, yeast cultures, low- ethanol wine
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