Production of poly(adenosine diphosphate-ribose) polymerase-1: development of an efficient production and purification protocol

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Knight, Matthew (2003) Production of poly(adenosine diphosphate-ribose) polymerase-1: development of an efficient production and purification protocol. PhD thesis, Victoria University of Technology.

Abstract

PARP-1 is a nuclear enzyme involved in a range of activities associated with DNA metabolism, playing a key role in maintaining the integrity of DNA and chromatin structure. As such, this enzyme is likely to provide a useful target when using a rationale drug design approach to develop pharmaceutical reagents including cancer therapeutics. A major obstacle to this work however is that our knowledge of the relationship between structure and function of PARP-1 is rather limited. Structure - function studies of enzymes typically require the application of techniques for producing reasonable quantities of high quality protein. To this end, this thesis describes the development of a rapid and relatively simple approach to producing and purifying PARP-1.

Item type Thesis (PhD thesis)
URI http://vuir.vu.edu.au/id/eprint/15621
Subjects Current > FOR Classification > 0601 Biochemistry and Cell Biology
Historical > Faculty/School/Research Centre/Department > School of Engineering and Science
Current > FOR Classification > 0304 Medicinal and Bimolecular Chemistry
Keywords NAD-ADP-ribosyltransferase, Separation, Purification, enzymes, DNA, PARP-1
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