Identification and characterisation of bacterial genes associated with resistance to and/or degradation of environmental pollutants

Davis, Belinda L. (2011) Identification and characterisation of bacterial genes associated with resistance to and/or degradation of environmental pollutants. PhD thesis, Victoria University.

Abstract

Bacteria were previously isolated from two separate sites, one contaminated with lead and the other with Polycyclic Aromatic Hydrocarbons (PAHs). Alcaligenes sp. AO22 and Arthrobacter sp. E9 were identified from the lead contaminated site, while Stenotrophomonas maltophilia was identified from the PAH contaminated site. Minimum Inhibitory Concentration assays (MICs) were previously performed on Alcaligenes sp. AO22 and Arthrobacter sp. E9 and they were found to be resistant to varying levels of heavy metals and polymerase chain reactions suggesting the presence of mercury (mer), copper (pco) and cadmium, zinc and cobalt (czc) resistance genes. S. maltophilia VUN 10010, was previously investigated for its ability to degrade pyrene and other HMW PAHs as a sole carbon and energy source. The purpose of the current project was to further characterise the MICs of these isolates, along with biofilm capabilities. The genetic basis of their heavy metal resistance was also investigated.

Item type Thesis (PhD thesis)
URI http://vuir.vu.edu.au/id/eprint/16038
Subjects Current > FOR Classification > 0399 Other Chemical Sciences
Current > FOR Classification > 0604 Genetics
Historical > Faculty/School/Research Centre/Department > School of Engineering and Science
Keywords Bacterial genes, gene identification, environmental pollutants, environmental contaminants, toxicity,genetics, environmental pollution, environmental contamination, resistant genes, microbial resistance, heavy metal resistance, PAH degradation, contamination, heavy metals, polymerase chain reactions , lead, cadmium, mercury, zinc, cobalt, mercury resistance, cadmium resistance, lead resistance, zinc resistance, cobalt resistance, Polycyclic Aromatic hydrocarbons, PAHs, 16S RNA, Alcaligenes sp. AO22, Achromobacter sp. AO22 , Arthobacter sp. E9, A. Woluwensis, Stenotrophomonas maltophilia, Minimum Inhibitory Concentration assays, MICs, S. maltophilia VUN 10010, pyrene, HMW PAHs, Mycobacteriu, methylmercury chloride, phenylmercury acetate, mer genes, organomercurial lyase, HgCl2, Reverse Transcriptase PCR, RT-PCR, HgCl2, biofilms, pseudomonas aeruginosa, chloride, nitrate, pbr operon, C. metallidurans CH34, cad operon, S. aureus pI258, Consortium VUN 10010
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