Isolation and characterisation of cDNAs encoding protein disulphide isomerases and cyclophilins in wheat

Full text for this resource is not available from the Research Repository.

Johnson, Joshua ORCID: 0000-0003-0000-5226, Clarke, B. C and Bhave, Mrinal (2001) Isolation and characterisation of cDNAs encoding protein disulphide isomerases and cyclophilins in wheat. Journal of Cereal Science, 34 (2). pp. 159-171. ISSN 0733-5210


The enzyme families of protein disulphide isomerases (PDI) and cyclophilins catalyse the isomerisation of disulphide bonds and the rotation of Xaa-Pro peptide bonds in nascent proteins, respectively, making them excellent candidates for regulating the deposition of storage proteins in wheat. This study aimed to isolate and characterise clones encoding cyclophilins and PDIs from a wheat endosperm cDNA library. A number of cDNA clones were isolated, of which three different cDNAs each of PDI and cyclophilin were selected for complete sequencing. The three PDI cDNAs encoded putative protein products of 513 or 516 amino acids, all exhibiting conserved sequences for the N-terminal signal peptide, the two thioredoxin-like domains at the catalytic sites and the C-terminal ER retention signal. The three cyclophilin cDNAs exhibited 68–87% identity to other reported cyclophilins and encoded putative protein products of 171 amino acids containing the conserved tryptophan residue and a 7 amino acid sequence unique to certain plant cyclophilins. The sequence variations within and outside the coding regions of all the cDNAs suggest that both enzymes are encoded by multiple genes. This information will allow further investigations into the roles of these enzymes in storage protein deposition in the developing endosperm and thus in wheat quality.

Dimensions Badge

Altmetric Badge

Item type Article
DOI 10.1006/jcrs.2001.0382
Official URL
Subjects Historical > RFCD Classification > 290000 Engineering and Technology
Historical > Faculty/School/Research Centre/Department > School of Engineering and Science
Keywords protein disulphide isomerase, peptidyl prolyl cis-trans isomerase, cyclophilin A, wheat quality
Citations in Scopus 27 - View on Scopus
Download/View statistics View download statistics for this item

Search Google Scholar

Repository staff login