Kostraby, Margaret M, Smallridge, Andrew John and Trewhella, Maurice Arthur (2002) Yeast-mediated preparation of I-PAC in an organic solvent. Biotechnology and Bioengineering, 77 (7). pp. 827-831. ISSN 0006-3592

Abstract

The yeast-mediated acyloin condensation of benzaldehyde and pyruvic acid to form l-PAC occurs in a petroleum spirit solvent system at room temperature with moderate conversion (30%) and high enantioselectivity (86%ee) after 24 h. The addition of a small amount of ethanol (0.5% mL) to the reaction mixture inhibits the formation of the side product benzyl alcohol and increases the conversion to l-PAC. Conducting the reaction using 13C-labeled pyruvate indicated that the pyruvate was incorporated into the l-PAC and that the excess pyruvate was converted into ethanol. Conducting the reaction at 5°C results in similar conversion but higher enantioselectivity.

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