a-Tocopheryl phosphate: a novel, natural form of vitamin E

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Gianello, Robert, Libinaki, Roksan, Azzi, Angelo, Gavin, Paul D, Negis, Yesim, Zingg, Jean-Marc, Holt, Phillip, Keah, Hooi-Hong, Griffey, Annike, Smallridge, Andrew John, West, Simon M and Ogru, Esra (2005) a-Tocopheryl phosphate: a novel, natural form of vitamin E. Free Radical Biology & Medicine, 39 (7). pp. 970-976. ISSN 0891-5849


We have detected a-tocopheryl phosphate in biological tissues including liver and adipose tissue, as well as in a variety of foods, suggesting a ubiquitous presence in animal and plant tissue. a-Tocopheryl phosphate is a water-soluble molecule that is resistant to both acid and alkaline hydrolysis, making it undetectable using standard assays for vitamin E. A new method was therefore developed to allow the extraction of both a-tocopheryl phosphate and a-tocopherol from a single specimen. We used ESMS to detect endogenous a-tocopheryl phosphate in biological samples that also contained a-tocopherol. Due to the significance of these findings, further proof was required to unequivocally demonstrate the presence of endogenous a-tocopheryl phosphate in biological samples. Four independent methods of analysis were examined: HPLC, LCMS, LCMS/MS, and GCMS. a-Tocopherol phosphate was identified in all instances by comparison between standard a-tocopheryl phosphate and extracts of biological tissues. The results show that a-tocopheryl phosphate is a natural form of vitamin E. The discovery of endogenous a-tocopheryl phosphate has implications for the expanding knowledge of the roles of a-tocopherol in biological systems.

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Item type Article
URI https://vuir.vu.edu.au/id/eprint/1871
DOI 10.1016/j.freeradbiomed.2005.05.016
Official URL http://dx.doi.org/10.1016/j.freeradbiomed.2005.05....
Subjects Historical > Faculty/School/Research Centre/Department > School of Engineering and Science
Keywords a-tocopheryl phosphate, vitamin E phosphate, vitamin E, biological tissues, free radicals
Citations in Scopus 86 - View on Scopus
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