ACE-inhibitory activity of probiotic yoghurt

Full text for this resource is not available from the Research Repository.

Donkor, Osaana ORCID: 0000-0001-9565-9024, Henriksson, A, Singh, TK, Vasiljevic, Todor ORCID: 0000-0003-1395-7349 and Shah, Nagendra P (2007) ACE-inhibitory activity of probiotic yoghurt. International Dairy Journal, 17 (11). pp. 1321-1331. ISSN 0958-6946

Abstract

In this study, the in vitro angiotensin-converting enzyme (ACE)-inhibitory (ACE-I) activity of peptide fractions from different yoghurt batches was assessed. Inhibition of ACE activity resulted in an overall antihypertensive effect. Yoghurts were prepared either using a sole yoghurt culture including Lactobacillus delbrueckii ssp. bulgaricus Lb1466 and Streptococcus thermophilus St1342, or L. acidophilus L10, L. casei L26 and Bifidobacterium lactis B94 in addition to yoghurt culture. ACE-I activity was determined at weekly intervals during 28 days of cold storage. Peptide fractions showing high ACE-I activity were further purified using multiple-steps of RP-HPLC. All probiotic yoghurts showed appreciable ACE-I activity during initial stages of storage compared with the control yoghurt, with a significant (p<0.05) decrease afterwards. The ACE-I activity ranged from IC50 of 103.30–27.79 μg mL−1 with the greatest ACE inhibition achieved during first and third week of storage. The in vitro ACE-I activity could be related to the peptide liberation via degradation of caseins. In total, 8 ACE-I peptides were characterized originating from αs2-casein (1), κ-casein (2) and β-casein, of which two well-known ACE-inhibiting peptides, namely Val–Pro–Pro (VPP) and Ile–Pro–Pro (IPP), were identified. These peptides are already used in commercial products.

Dimensions Badge

Altmetric Badge

Item type Article
URI https://vuir.vu.edu.au/id/eprint/1872
DOI https://doi.org/10.1016/j.idairyj.2007.02.009
Official URL http://dx.doi.org/10.1016/j.idairyj.2007.02.009
Subjects Historical > RFCD Classification > 210000 Science-General
Historical > Faculty/School/Research Centre/Department > School of Engineering and Science
Keywords yoghurt, bioactive peptides, angiotensin I-converting enzyme, ACE inhibition
Citations in Scopus 174 - View on Scopus
Download/View statistics View download statistics for this item

Search Google Scholar

Repository staff login