Short-Term Intensified Cycle Training Alters Acute and Chronic Responses of PGC1 Alpha and Cytochrome C Oxidase IV to Exercise in Human Skeletal Muscle

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Stepto, Nigel ORCID: 0000-0002-0875-6836, Benziane, Boubacar, Wadley, Glenn D, Chibalin, Alexander V, Canny, Benedict J, Eynon, Nir ORCID: 0000-0003-4046-8276 and McConell, Glenn ORCID: 0000-0002-8572-9065 (2012) Short-Term Intensified Cycle Training Alters Acute and Chronic Responses of PGC1 Alpha and Cytochrome C Oxidase IV to Exercise in Human Skeletal Muscle. PLoS ONE, 7 (12). ISSN 1932-6203

Abstract

Reduced activation of exercise responsive signalling pathways have been reported in response to acute exercise after training; however little is known about the adaptive responses of the mitochondria. Accordingly, we investigated changes in mitochondrial gene expression and protein abundance in response to the same acute exercise before and after 10-d of intensive cycle training. Nine untrained, healthy participants (mean plus or minus SD; VO2peak 44.1 plus or minus 17.6 ml/kg/min) performed a 60 min bout of cycling exercise at 164 plus or minus 18 W (72% of pre-training VO2peak). Muscle biopsies were obtained from the vastus lateralis muscle at rest, immediately and 3 h after exercise. The participants then underwent 10-d of cycle training which included four high-intensity interval training sessions (6x5 min; 90–100% VO2peak) and six prolonged moderate-intensity sessions (45–90 min; 75% VO2peak). Participants repeated the pre-training exercise trial at the same absolute work load (64% of pre-training VO2peak). Muscle PGC1-alpha mRNA expression was attenuated as it increased by 11- and 4- fold (P<0.001) after exercise pre- and post-training, respectively. PGC1-a protein expression increased 1.5 fold (P<0.05) in response to exercise pre-training with no further increases after the post-training exercise bout. RIP140 protein abundance was responsive to acute exercise only (P<0.01). COXIV mRNA (1.6 fold; P<0.01) and COXIV protein expression (1.5 fold; P<0.05) were increased by training but COXIV protein expression was decreased (20%; P<0.01) by acute exercise pre- and post-training. These findings demonstrate that short-term intensified training promotes increased mitochondrial gene expression and protein abundance. Furthermore, acute indicators of exercise-induced mitochondrial adaptation appear to be blunted in response to exercise at the same absolute intensity following short-term training.

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Item type Article
URI https://vuir.vu.edu.au/id/eprint/21734
DOI https://doi.org/10.1371/journal.pone.0053080
Official URL http://www.plosone.org/article/info%3Adoi%2F10.137...
Subjects Historical > Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
Current > FOR Classification > 0604 Genetics
Current > FOR Classification > 1106 Human Movement and Sports Science
Historical > Faculty/School/Research Centre/Department > School of Sport and Exercise Science
Historical > Faculty/School/Research Centre/Department > Institute of Sport, Exercise and Active Living (ISEAL)
Keywords ResPubID25580, e53080, ∞, +, -, less than, intense exercise, cycling, training, proteins, RNA extraction, RT-QPCR, antibodies, reagents, gene expression, protein expression, muscle protein extraction, immunoblot analysis, muscles, ETS mitochondrial proteins, regulation of protein abundance
Citations in Scopus 39 - View on Scopus
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